Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia



Makarkina Marina


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Articles in journal: (total 26)

pdf
587 Кб
11 с.
Genetic Resources, Cultivar, selection
Date posted: 15.01.2020
UDC: 575.11: 634.84
DOI: 10.30679/2219-5335-2020-1-61-33-43
Keywords: VITIS VINIFERA GENOTYPES, LOCAL GRAPE CULTIVARS OF ABKHAZIA, SSR-LOCI, DNA-FINGERPRINT

Annotation

Traditional ampelographic descriptions are supplemented by molecular genetic data in the study of the grape gene pool at the present time. The territory of Abkhazia is one of the centers of the cultivated grapes arising; many wild-growing forms and ancient native grape varieties are found here. Local native cultivars of different regions are the important part of world grape gene pool of Vitis vinifera. We performed the genotyping of Kachich grape plants, which can be qualify as the most famous local variety of Abkhazia. SSR markers recommended by the Union of Grape Genetics for genotyping of Vitis vinifera were used in the work: VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79. Molecular genetic analysis was carried out performed by PCR using an ABI Prism 3130 automatic genetic analyzer. The objects of study are grape plants cultivated at three points in Abkhazia under the name Kachich and have morphological characteristics similar to the variety described in the USSR Ampelography. DNA analysis revealed that one of the analyzed samples is significantly different from the other two samples in microsatellite profiles. Thus, it can be argued that, in general, at least two different genotypes with similar morphological characteristics are grown under one name in Abkhazia, and, two other samples of the variety showed identical DNA profiles of plants. According to ampelographic features, these samples are closed to the description of the Kachich morphotype presented in ampelography. One of these samples grows in the collection of Wines and Waters of Abkhazia company and were planting with planted material from the ampelographic collection of the Research Institute of Viticulture and Winemaking Magarach. It can be concluded that we obtained the DNA profile of the Kachich genotype corresponding to the morphological description of the variety.

How to cite
Ilnitskaya E., Makarkina M., Tokmakov S., Krasilnikov A., Aiba V., Avidzba M. GENOTYPING OF GRAPE PLANTS OF KACHICH VARIETY FROM DIFFERENT PLACES OF GROWING [Electronic resource] // Fruit growing and viticulture of South Russia. 2020. № 61(1). pp. 33–43. URL: http://journalkubansad.ru/pdf/20/01/03.pdf. DOI: 10.30679/2219-5335-2020-1-61-33-43 (request date: 29.03.2024).
pdf
485 Кб
10 с.
Genetic Resources, Cultivar, selection
Date posted: 15.01.2020
UDC: 634.84.09: 575.222.72: 575.113
DOI: 10.30679/2219-5335-2020-1-61-44-53
Keywords: GRAPES, DOWNY MILDEW RESISTANSE, VITIS AMURENSIS, DNA-MARKERS, RPV10, RPV12

Annotation

The main grape varieties used as table varieties and for winemaking belong to the species Vitis vinifera L., which has a high quality of berries, but rather low resistance to diseases and frost. One of the donors of valuable features f or the breeding of grape varieties is the species of Vitis amurensis Rupr. For example, some V. amurensis genotypes carry the downy mildew resistance genes of Rpv10 and Rpv12. The aim of the work is to study the genetic potential of resistance to downy mildew by DNA-marker analysis of some hybrid forms obtained using Amur grapevines. The research was carried out on the DNA of twenty-one grape genotypes, created by F.I. Shatilov using V. amurensis, and preserved in the collection of A.Y. Nemytov (Orenburg Region). DNA extraction was carried out by CTAB method from herbalized young shoots of grape plants. In the work for identification gene Rpv10 the closely linked DNA marker GF09-46 was used to identify the Rpv12 gene markers UDV360, UDV343, flanking the locus of resistance. Separation of reaction products and analysis of PCR fragment size were carried out using an automatic genetic analyzer. As control genotypes in the study the grape varieties those carriers the genes of Kunlean (Rpv12) and Saperavi Severnyi (Rpv10) were included. In two forms under the conventional names Muscat Severniy No. 1 and Hybrid form No. 3 the presence of genes Rpv12 and Rpv10 were revealed, respectively. These genotypes have a number of positive characteristics that are promising in the breeding of table grape varieties and can be considered as the sources o f valuable traits.

How to cite
Ilnitskaya E., Makarkina M., Tokmakov S., Kotlyar V., Nemytov A. DNA-MARKER STUDY OF THE GENETIC POTENTIAL OF DOWNY MILDEW RESISTANCE IN HYBRID FORMS FROM VITIS AMURENSIS [Electronic resource] // Fruit growing and viticulture of South Russia. 2020. № 61(1). pp. 44–53. URL: http://journalkubansad.ru/pdf/20/01/04.pdf. DOI: 10.30679/2219-5335-2020-1-61-44-53 (request date: 29.03.2024).
pdf
132 Кб
9 с.
Breeding and production of planting material
Date posted: 15.03.2013
UDC: 001.4:632.3
Keywords: ORCHARD NURSERY, PRODUCTION OF FRUIT CROPS SAPLINGS, ASSORTMENT, PHYTOSANITARY SITUATION, MECHANIZATION

Annotation

The analysis of fruit crops saplings production in the Krasnodar region for 5 last years is presented in the article. Producer structure, the phytosanitary situation and cultivar policy in the orchard nursery are analised. The problems of the modern fruit crops saplings production in the region are allocated and the possible ways of these problems decision are given.

How to cite
Buntsevich L., Kostyuk M., Paletskaya E., Makarkina M. PHYTOSANITARY SITUATION AND CULTIVAR POLICY IN THE ORCHARD NURSERY OF KRASNODAR REGION [Electronic resource] // Fruit growing and viticulture of South Russia. 2013. № 20(2). pp. 47–55. URL: http://journalkubansad.ru/pdf/13/02/05.pdf. (request date: 29.03.2024).
pdf
668 Кб
8 с.
Genetic Resources, Cultivar, selection
Date posted: 15.03.2018
UDC: 575.11: 634.8.09
DOI: 10.30679/2219-5335-2018-2-50-24-31
Keywords: GRAPEVINE, DNA-MARKERS, DOWNY MILDEW RESISTANCE, RPV10, RPV3 GENES

Annotation

Downy mildew is the one of the most prevalent diseases of the grapevine. An effective way to control the spread of disease is cultivation of resistant varieties. The European grape V. vinifera is not resistant to mildew practically. Genotypes, carrying the resistance genes, belong to the grape varieties of North America and Asia. So breeding of resistant grape varieties is based on interspecies hybridization. Modern methods of genetics and molecular biology expand the possibilities of ongoing research in this field. Today it is identified about 20 major and minor of resistance loci to P. viticola. Rpv10 and Rpv3 are major loci of resistance. Rpv10, inherited from V. amurensis was first determined F. Schwander et al. It was identified and a DNA-marker GF09-46, closely linked with this locus. Rpv3 gene was first found in the Bianca grapes a complex interspecies hybrids of grapes. It was later determined that the Rpv3 gene has seven haplotypes, and their identification is possible with microsatellite markers UDV305, UDV737. We analyzed the genotypes of selected, elite forms and new inter-species grape varieties of NCFSCHVW breeding by the PCR method using DNA markers linked to the mildew resistance Rpv10 and Rpv3 genes. The separation of the reaction products is carried out by capillary electrophoresis using an automated genetic analyzer ABI Prism 3130. The analyzes were carried out in accordance with the potential possibility of identifying the target alleles according to the pedigree of the studied samples. The Rpv3 gene is defined in six hybrid genotypes, Rpv10 gene is defined in one grape hybrid.

How to cite
Ilnitskaya E., Tokmakov S., Makarkina M. DNA MARKER-ASSISTED IDENTIFICATION OF RESISTANCE GENES TO DOWNY MILDEW IN NEW CULTIVARS AND SELECTED GRAPES FORMS BY NCFSCHVW BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2018. № 50(2). pp. 24–31. URL: http://journalkubansad.ru/pdf/18/02/03.pdf. DOI: 10.30679/2219-5335-2018-2-50-24-31 (request date: 29.03.2024).
pdf
480 Кб
13 с.
Genetic Resources, Cultivar, selection
Date posted: 15.05.2020
UDC: 634.84.09: 575.222.72: 575.113
DOI: 10.30679/2219-5335-2020-3-63-1-13
Keywords: GRAPES, DNA-CERTIFICATION, PATHOGENS, IDENTIFICATION, REAL-TIME PCR, DEEP SEQUENCING

Annotation

Grapes are a valuable cultural plant for human. It is used fresh, as a raw material for the juice, wine and canning industries, and for the various types of dried products. Of the total number of grapes produced in the world, 80-90 % is used for processing into wines, juices and other products, up to 10 % of the grapes are consumed fresh and 5-6 % goes to drying. Industrial management of high-quality grape plantations and nurseries is impossible without the use of scientific knowledge that allows you to identify the most productive grape varieties for specific agro-climatic zones, determine the purity of plants and the diagnose the phytopathogens in the planting material and existing plantations. One of the most effective methods for solving problems of nursery management is molecular genetic, which are widely used for DNA certification, determining the origin of grapes and for identifying pathogens in the planting material. DNA profiling allows us to screen out mutant forms at an early stage or select them for further research. Diagnostics of pathogens in the uterine plantations and planting material includes the identification of pathogenic organisms and early detection of asymptomatic diseases (viruses, phytoplasmic infections, tracheomycosis, bacterial cancer). Methods of control of planting material, which are widely used in nursery management, include the enzyme immunoanalisis, placement the indicator plants into the uterine plantations, PCR method and deep sequencing. The use of these methods in the nursery allows to correctly identify the varietal identity of the material on the early stages, to identify the presence of pathogens harmful, and make timely set of protective measures that later will ensure the effective development and high quality of the uterine plants.

How to cite
Kotlyar V., Ilnitskaya E., Makarkina M., Lobodina E. USE OF MOLECULAR GENETIC METHODS FOR VARIETAL IDENTIFICATION AND ACCELERATED DIAGNOSTICS OF PHYTOPATHOGENS LATENT FORMS IN THE PROBLEMS SOLVING OF GRAPE NURSERY [Electronic resource] // Fruit growing and viticulture of South Russia. 2020. № 63(3). pp. 1–13. URL: http://journalkubansad.ru/pdf/20/03/01.pdf. DOI: 10.30679/2219-5335-2020-3-63-1-13 (request date: 29.03.2024).