Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia



Odessa National University named after I.I. Mechnikov

Candidate of Physico-mathematical Sciences, associated professor

Articles in journal: (total 1)

pdf
202 Кб
10 с.
Breeding and production of planting material
Date posted: 15.01.2016
UDC: 634.8:631.532:631.544
Keywords: GRAPEVINE, CULTURE IN VITRO, NUTRIENT MEDIUM, METHOD OF MULTICRITERIAN OPTIMIZATION, GREAT NUMBER OF SHOOTS

Annotation

One way to improve the efficiency of grapes plants breeding in vitro is the method of induction of multiple shoots on various types of cultural medium. Applying this method, we can significantly increase in the multiplication factor of plants and, therefore, to achieve the increase in the production of planting material free from viral and bacterial infection. The aim of our study was to determine the optimal culture medium for the induction of multiple grapes shoots in vitro by applying the mathematical apparatus of multi-criterial optimization. Study was carried out in the laboratory of tissue culture V.E. Tairov "Institute of Viticulture and Winemaking" on grapes varieties of Mechta, Kobzar, Original. In order to optimize the composition of the nutrient medium for the formation of multiple shoots we tested the various medium options. To improve the efficiency of induction of multiple shoots of grapes plants in the culture in vitro was studied and proved the advantage of using semi-liquid medium in comparison with solid and liquid medium. When using the multi-criterion optimization methods it is determined the optimal nutrient medium, namely a modified semi-solid MS medium, which contributed to a better survival, differentiation, regeneration and productivity of meristems of grapes plants. It is shown that the introduction of an improved method of induction of multiple shoots helped to increase in the multiplication factor of grapes in vitro culture to 1: 9.5, and this method reduced the cost of micro clones of grapes in the early stages of micropropagation by 90%.

How to cite
Tesluk N., . THE USE OF METHOD OF MULTICRITERIAN OPTIMIZATION FOR EVALUATION OF INDUCTION PROCESS FOR THE GREAT NUMBER OF GRAPEVINE SHOOTS IN VITRO [Electronic resource] // Fruit growing and viticulture of South Russia. 2016. № 37(1). pp. 151–160. URL: http://journalkubansad.ru/pdf/16/01/12.pdf. (request date: 29.03.2024).