Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia



Avidzba Madina Anatolyevna

State Scientific Institution Institute of Agriculture of Sciences Academy Abkhazia

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Articles in journal: (total 1)

pdf
587 Кб
11 с.
Genetic Resources, Cultivar, selection
Date posted: 15.01.2020
UDC: 575.11: 634.84
DOI: 10.30679/2219-5335-2020-1-61-33-43
Keywords: VITIS VINIFERA GENOTYPES, LOCAL GRAPE CULTIVARS OF ABKHAZIA, SSR-LOCI, DNA-FINGERPRINT

Annotation

Traditional ampelographic descriptions are supplemented by molecular genetic data in the study of the grape gene pool at the present time. The territory of Abkhazia is one of the centers of the cultivated grapes arising; many wild-growing forms and ancient native grape varieties are found here. Local native cultivars of different regions are the important part of world grape gene pool of Vitis vinifera. We performed the genotyping of Kachich grape plants, which can be qualify as the most famous local variety of Abkhazia. SSR markers recommended by the Union of Grape Genetics for genotyping of Vitis vinifera were used in the work: VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79. Molecular genetic analysis was carried out performed by PCR using an ABI Prism 3130 automatic genetic analyzer. The objects of study are grape plants cultivated at three points in Abkhazia under the name Kachich and have morphological characteristics similar to the variety described in the USSR Ampelography. DNA analysis revealed that one of the analyzed samples is significantly different from the other two samples in microsatellite profiles. Thus, it can be argued that, in general, at least two different genotypes with similar morphological characteristics are grown under one name in Abkhazia, and, two other samples of the variety showed identical DNA profiles of plants. According to ampelographic features, these samples are closed to the description of the Kachich morphotype presented in ampelography. One of these samples grows in the collection of Wines and Waters of Abkhazia company and were planting with planted material from the ampelographic collection of the Research Institute of Viticulture and Winemaking Magarach. It can be concluded that we obtained the DNA profile of the Kachich genotype corresponding to the morphological description of the variety.

How to cite
Ilnitskaya E., Makarkina M., Tokmakov S., Krasilnikov A., Aiba V., Avidzba M. GENOTYPING OF GRAPE PLANTS OF KACHICH VARIETY FROM DIFFERENT PLACES OF GROWING [Electronic resource] // Fruit growing and viticulture of South Russia. 2020. № 61(1). pp. 33–43. URL: http://journalkubansad.ru/pdf/20/01/03.pdf. DOI: 10.30679/2219-5335-2020-1-61-33-43 (request date: 29.03.2024).