Fruit growing
and viticulture of South Russia

Annotation

The experimental data of estimation of efficiency of new generation of antibiotics for suppression of bacterial and fungus infection of nutrient mediums and rootstocks explants of series SK at their introduction in culture in vitro are presented. Experimental results of some sterilizers efficiency at introduction in culture in vitro of strawberry and blackberry explants are presented

Annotation

For modern clonal micro propagation the great interest is represented by low-toxic, environmentally friendly, budgetary growth regulators of new generation, in particular the derivatives of organic acids received when processing waste of agricultural production. These preparations pass the broad tests in agrarian production, but in vitro culture their test wasn't carried out. This work is performed for the purpose of study of plantlets' growth reactions and definition of an optimum ratio and concentration of new and traditional growth factors at a stage of regeneration and multiplication of plum micro shoots. As object of research the plum of Stanley variety is served. As a result of the conducted research it is revealed that on nutrient mediums where as a growth factor amber acid 4 mg/l was used, the reproduction coefficient for a passage (3,5) is the same, as well as on the medium with standard stimulators - BAP 0,2 mg/l and GA 1,2 mg/l, a little smaller coefficient of reproduction (3,3) - on the medium with potassium succinate. Use of amber acid, potassium succinate and sodium succinate in concentration 4 mg/l considerably reduces the level of micro shoots' chlorosis (by 53,9, 48 and 31,5% respectively), improves their general state (on 1,3; 1,2 and 0,9 points on a five-point scale respectively). The using of amber acid and a sodium succinate 4 mg/l significantly increases the quantity of the formed leaves on microshoots, on 4,1 - 4,4 pieces, in comparison with the standard (BAP 0,2 mg/l and GA 1,2).

Annotation

The purpose of the research was to obtain a preparative form of extractive cell culture "in vitro" of ginseng in the laboratory conditions, as well as in the development of products based on ginseng extracts. In the article the results of own research are used and analytical review of domestic and foreign sources. The work is performed in the laboratory of biotechnology and the center of winemaking of North Caucasian Regional Research Institute of Horticulture and Viticulture. The object of research and improvement of methods of production of ginseng has been in vitro culture. In the study the biomaterial of ginseng and extract of roots are used. The research techniques are generally accepted. In the article the useful properties of a ginseng and problems of its cultivation in garden culture are given. The actuality of receiving of its biomass in vitro is specified. The structure of a nutrient medium for cultivation of a ginseng's cellular culture is developed: the macro � and microelements according to Murashige and Skoog, sucrose � 30 mg/l, tiamin-chloride � 1 mg/l, mesoinositol � 80 mg/l, kinetin � 1 mg/l, α-��� � 2 mg/l, iron chelate � 10 mg/l, an agar-agar � 7 mg/l. The ginseng�s biomass cultivation cycle is characterized. The flavouring-aromatic addition of "Zhenshenevaya" is made, its organoleptic and physical and chemical features are given. As one of the ginseng preparations, this addition inherits the characteristics of a ginseng. It possesses the toning property and positively influences on person's condition when a hypotonia, an exhaustion, a neurasthenia, the lowered potentiality. It is a fine adaptogen, which increases the organism resistance to diseases and stresses. On the basis of this addition the unique aerated and not aerated soft drinks, low alcohol and hard drinks as, vodka, balms, liquors are developed.

Annotation

The urgency of stud� is caused by the high economic value of Sharkey's virus (PPV), determined by the losses of productivity and quality of plum fruits. In receptive plum varieties also of other stone fruit crops the infection by Sharkey's virus leads to shedding of the fruits, and ulcers on the surface of fruits. Virus infection is manifested symptomatically in tolerant plants, but does not affect, to the expressed degree the anatomical-morphological and functional state of the infected organisms. The purpose of this work was the study of the plum tolerance to Sharkey's virus (PPV) and the revealing of regularities of the change in the receptivity of plum to this virus in the course of reproduction by the method of inoculation (budding). In the experiment there are three plum varieties: Stanley, Donetskaya and Kubanskaya Rannia. In the process of experiments the regeneration of buds from the infected symptomatic and infected asymptomatic mother trees is studied. Virus carrying of the plum plants is confirmed by OT-PCR testing. The results show that the buds from the symptomatic mother plum trees, virus carriers PPV, survive more badly than buds from the asymptomatic mother trees, also the virus PPV carriers. The level of survival in the version with the buds from the symptomatic trees on the average to 17% lower than those of the version with the asymptomatic trees, and to 27 % lower than in the control version with the virus-free trees. The reason for reduction in the survival of buds infected Sharkey's virus of plum on the virus-free rootstock is the property of Sharkey's virus to cause the corking of the undifferentiated plant tissues. During ripening of fruits, for instance, this property of plum Sharkey leads to premature shaping of the separating layer between the leafstalk and shoot, that in turn, causes the shedding of fruits (the month before the ripening).

Annotation

Clonall micro reproduction is widely used in the breeding of fruit and berry crops for reproduction of a small number of genotypes and for a fast receiving a large amount of virus-free material. The object of research is the strawberry apexes from 1 to 3 mm in size and the meristems (0,3 mm) of Klery, Asia, Alba and Marmolada varieties. The influence of some factors of strawberry cultivation at a stage of explants introduction in vitro is revealed in the article. As a result of the carried out research it is established that input in vitro of apexes of 3 mm in size, with the subsequent exarticulation from them of meristems of 0,3 mm in size, increases of explants survival (meristems of 0,3 mm) more than twice (from 25% to 59% on average). It is shown that the efficiency of clonal strawberry micro reproduction is increased on the nutrient mediums containing a complex 6-BAS with IAA in the ratio 1:0,1. This medium provides the high coefficient of reproduction together with high quality of regenerated micro shoots. It is also established that the structure of medium with complex of 6-BAS and detiobiotine causes more intensive growth of shoots and activates of chlorophyll synthesis. The darkening of explants for 8 days at the 1 st passage leads to increase in coefficient of reproduction of Asia strawberry explants from 22,6 to 64,3 at the medium containing 6-BAS without IAA and to increase in coefficient of reproduction from 9,4 to 16,0 of Alba strawberry. In addition in the medium of 0,1 mg/l of IAA the coefficient of reproduction increases from 33,2 to 40,0 at Asia strawberry and from 12,6 to 16,4 at Alba strawberry.