Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia



Ilnitskaya Elena


Candidate of Biological Sciences

Articles in journal: (total 42)

pdf
584 Кб
13 с.
Genetic Resources, Cultivar, selection
Date posted: 27.03.2023
UDC: 634.8: 575.113.2
DOI: 10.30679/2219-5335-2023-2-80-48-60
Keywords: GRAPE VARIETY, GENOTYPE, DNA MARKERS, DNA PASSPORT, ORIGIN CLARIFICATION

Annotation

The use of DNA markers, along with classical identification methods based on morphological assessment, makes it possible to more accurately determine the varietal affiliation of grape plants, as well as clarify the origin of genotypes. The purpose of this work is to perform genotyping of grape varieties bred by the Dagestan Breeding Experimental Station of Viticulture and Vegetable Growing (DBESV&V) using microsatellite markers (SSR) used for varietal identification and clarify the origin of varieties using DNA analysis data. The objects of the study were six table grape varieties Vezne, Dolchatyi, Zhemchuzhina Yuga, Zarya Derbenta, Leki and Muscat Derbenskiy. The DNA of the studied varieties was extracted by the CTAB method from herbarized plant material. Genotyping was carried out using a standard set of SSR markers: VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79 by PCR followed by analysis of the results on an ABI Prism 3130 genetic analyzer, the obtained data were processed using the GeneMapper v 4.1 program and aligned with reference variety Pinot noir. Pedigrees of varieties were analyzed based on the principle of codominant inheritance of alleles from parents to offspring. DNA profiles of parental forms (Csaba gyoengye, Muscat of Alexandria, Muscat Hamburg, Kirovabadskiy stolovyi) of the studied varieties were taken from the international database of grape varieties VIVC (Vitis International Variety Catalogue), and we also used the previously developed DNA passport of Agadai variety. As a result of the study, DNA-passports of 6 varieties of DBESV&V breeding were developed. DNA analysis confirmed origin of grape varieties Vezne, Dolchatyi, Zhemchuzhina Yuga, Zarya Derbenta, Leki and Muskat Derbentskiy from the declared parental forms.

How to cite
Makarkina M., Ilnitskaya E., Kozina T., Kozhevnikov E., Kazahmedov R. DNA-IDENTIFICATION AND ANALYSIS OF PEDIGREE OF GRAPE VARIETIES DBESV&V BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2023. № 80(2). pp. 48–60. URL: http://journalkubansad.ru/pdf/23/02/04.pdf. DOI: 10.30679/2219-5335-2023-2-80-48-60 (request date: 28.03.2024).
pdf
417 Кб
17 с.
Processing of fruit and berries production and grapes
Date posted: 27.09.2023
UDC: 634.8.076; 663.253
DOI: 10.30679/2219-5335-2023-5-83-188-204
Keywords: BREEDING FORMS OF GRAPES, NEW GRAPE VARIETIES, WINE QUALITY, AROMA-FORMING COMPLEX OF WINE

Annotation

The current area of research is a comprehensive study of varieties, forms and clones of local breeding for a reasonable selection of modern varieties. The aim of the study was to study the biochemical and technological characteristics of varieties, forms and clones of grapes bred by NCFSCHVW in the edaphoclimatic conditions of the Kuban. The objects of research were varieties, forms, clones of the NCFSCHVW breeding (9 white-berry, 11 dark-berry and 4 control varieties), as well as fresh grape must and dry wine materials obtained from them. Wine materials were prepared according to classical technologies for low-oxidized white wines and red wines with a floating cap. The mass concentrations of sugars and titratable acids were determined in the must. Physicochemical and organoleptic characteristics, indicators, composition of aroma-forming components and individual organic acids were determined in wine materials. A complex of phenolic substances, vitamins and vitamin-like substances was studied in red wine materials. It was established that the must of the studied samples had moderate mass concentrations of sugars and titratable acids: white berry 19.1-22.5 g/100 cm3 and 7.6-8.3 g/dm3 , respectively; dark berries 20.2-24.4 g/100 cm3 and 6.8-8.0 g/dm3 , respectively. It was noted that the group of Tana forms was distinguished by a higher volume fraction of ethyl alcohol of 11.8-12.5 % vol. In a sample of dry wine material Tana 95, a higher content of esters (89.8 mg/dm3 ) was noted, among red ones in wine materials Tana 33 (116.8 mg/dm3 ), Kurchanskiy (100.1 mg/dm3 ) and Cabernet Kuban (105.2 mg/dm3 ). The mass concentration of higher alcohols in dry wine materials from the white berry forms of Tana (295.9-349.8 mg/dm3 ) exceeded the classic varieties and proto-clones from the Chardonnay and Riesling lines (192.6-264.4 mg/dm3 ). Vladimir stood out by the amount of phenolic substances (3603 mg/dm3 ), Tana 85 distinguished according to the accumulation of anthocyanins. Kurchanskiy, Vladimir and Tana 85 were isolated according to the amount of vitamins and vitamin-like substances.

How to cite
Shelud'ko O., Prakh A., Chemisova L., Ilnitskaya E. BIOCHEMICAL AND TECHNOLOGICAL FEATURES OF GRAPE VARIETIES, FORMS AND CLONES OF FSBSI NCFSCHVW BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2023. № 83(5). pp. 188–204. URL: http://journalkubansad.ru/pdf/23/05/15.pdf. DOI: 10.30679/2219-5335-2023-5-83-188-204 (request date: 28.03.2024).