Fruit growing
and viticulture of South Russia

Annotation

Research was conducted to identify and study the technological characteristics of autochthonous strains of wine yeast isolated from vineyards in the Krasnodar region. From natural populations, 90 yeast strains were isolated from the surface of grapes of the following varieties: Petit Mansan, Merlot, Krasnostop Anapskiy. Generic identification of the studied strains was carried out. The proportion of Saccharomycetes varied over a wide range. Analysis of 90 yeast strains made it possible to isolate 40 representatives of the genus Saccharomyces. Species identification was carried out using molecular genetic methods. A working sample of 40 strains of Saccharomyces cerevisiae was obtained. Yeast screening for the ability to ferment sugars made it possible to isolate 13 yeast strains. Strains TK20-2 and LM19-19 are noted as weak fermenters with the formation of non-fermenters. An analysis of the ability of yeast to synthesize hydrogen sulfide showed that 21 strains produce hydrogen sulfide in an insignificant amount. In the course of studying the main technological properties, 4 strains were isolated, with the use of which white wine materials were prepared from the Onitskanskiy grape variety. In wine materials produced using local strains, a variation in the concentrations of organic acids, amino acids and volatile aroma-forming components was noted. The conducted tasting of the studied samples showed that 3 out of 4 wine materials were characterized by high quality. The sample produced using strain LM19-3 had the highest tasting score. The use of strains LP19-1, TK20-11 and LM19-3 ensures the production of high-quality wine materials with high organoleptic characteristics.

Annotation

Syringa is a genus of about 27 wild species of flowering woody plants of the Oleaceae family. Syringa vulgaris L. is known with a wide variety of decorative varieties and hybrids suitable for use in urban and rural landscapes. In the botanical gardens and nurseries, the lilac is propagated with green cuttings or grafts, but all of its varieties do not reproduce well by these ways. There are difficulties in propagating the most decorative varieties. The production of a large number of varietal plants is limited by the season, the process of grafting, cutting and getting shoots is labor-intensive, and requires the presence of uterine plantations. Therefore, there is a need for development ways to propagate lilac that would be universal for all varieties and would create a cost-effective technology of fast reproduction of planting material. In vitro methods have opened up the new areas of research that can overcome the problems of traditional methods and ensure the rapid plants propagation on an industrial scale. Lilac plants obtained by microclonal propagation have several advantages over drafted ones. They are more lasting, decorative, quickly develop and bloom, and form the ideal habitus of the bush. In vitro plants have the juvenility signs, which allow them to be successfully propagated by other vegetative methods, especially by green cuttings. Objects of research are lilac varieties: Geisenkalis, Primrose, Marie Frances, Krasavitsa Moskva, Sensatsiya. The article presents the results of influence of the selection date (04/22/19 and 05/15/19) the contamination nation and the survival rate of five lilac varieties explants. The analysis have been shown that the contamination of the microshoots selected on April 22, 2019 was significantly less than that for the microshoots selected on May 15, 2019 and amounted to 37.4 and 67,2 %, respectively. The survival rate of explants was 57,7 % and 19,2 %.

Annotation

The intensive production of fruits, directed on obtaining of stable and high-quality crops, causes the necessity to increase the resistance of cultivars to diseases. Under the modern conditions of rapid climat change, caused by anthropogenic, including agricultural environmental contamination, the protective and adaptive characteristics of the plants are reducing, and this factor determines the need to obtain the varieties of fruit crops adapted to a wide range of changing external conditions both abiotic and biotic. The article presents the data of early apple seedling selection for resistance to the two apple-tree dominant fungal infections: to scab under artificial infestation and to powdery mildew on a natural background. The object of research is the hybrid apple seedlings resulting from deliberate crossbreeding of K15461 sample of MOSVIR collection, that is relatively steady to both pathogens, with the susceptible Fuji cultivar. In 36% of the samples a scab lesions were not identified, 39% of the seedlings are resistant to a powdery mildew, according to the two years of the evaluation. 3 seedlings showed the resistance to the two pathogens, their share is 11 %. The evaluation of the plant also on complex features "cultivation" made it possible to identify the most of them as significant for breeding. The obtained results indicate a high prospect of using of K15461, as well as hybrid plants obtained in the combination of crossing of K15461 × Fuji, as donors of resistance to scab and powdery mildew. The hybrid seedlings can be used for study directed to the identification and mapping of new genes for resistance of apple plants to these pathogens.

Annotation

Grapes are a valuable cultural plant for human. It is used fresh, as a raw material for the juice, wine and canning industries, and for the various types of dried products. Of the total number of grapes produced in the world, 80-90 % is used for processing into wines, juices and other products, up to 10 % of the grapes are consumed fresh and 5-6 % goes to drying. Industrial management of high-quality grape plantations and nurseries is impossible without the use of scientific knowledge that allows you to identify the most productive grape varieties for specific agro-climatic zones, determine the purity of plants and the diagnose the phytopathogens in the planting material and existing plantations. One of the most effective methods for solving problems of nursery management is molecular genetic, which are widely used for DNA certification, determining the origin of grapes and for identifying pathogens in the planting material. DNA profiling allows us to screen out mutant forms at an early stage or select them for further research. Diagnostics of pathogens in the uterine plantations and planting material includes the identification of pathogenic organisms and early detection of asymptomatic diseases (viruses, phytoplasmic infections, tracheomycosis, bacterial cancer). Methods of control of planting material, which are widely used in nursery management, include the enzyme immunoanalisis, placement the indicator plants into the uterine plantations, PCR method and �deep sequencing�. The use of these methods in the nursery allows to correctly identify the varietal identity of the material on the early stages, to identify the presence of pathogens harmful, and make timely set of protective measures that later will ensure the effective development and high quality of the uterine plants.

Annotation

In recent years, many wineries have chosen to avoid using commercial S. cerevisiae strains as fermentation starters, and preferred the use of autochthonous selected strains as an oenological strategy that allows combining the industrial advantages of inoculated fermentation with the authenticity and typicality of spontaneous fermentation by native S. cerevisiae strains. One of the important tasks in the search for autochthonous strains of S. cerevisiae is the assessment of the genetic identity of strains and their genetic diversity. The interdelta genotyping method is successfully used for this purpose. The paper presents the results of interdelta genotyping of 49 autochthonous S. cerevisiae strains isolated on white and red grape varieties on the territory of the Krasnodar region, using three primer pairs δ1+ δ2, δ12+ δ2, δ12+ δ21. It was found that when primer pairs δ12+ δ2 and δ12+ δ21 were used, 14 different patterns were formed in the samples. When using the primer pair δ1+ δ2 � 11 patterns. With the primer pair δ12+ δ21, 5 and 6 groups of genetic similarity were formed in the studied samples, which is higher than when using other types of δ pairs. According to the dendrogram obtained from the results of the interdelta analysis and constructed using the UPGMA method, the strains were divided into 2 main groups, and the first group included only yeasts isolated on the Dostoynyi grape variety (19 out of 28 strains). The second group included 9 strains isolated on the Dostoynyi grape variety and all strains isolated on the Chardonnay grape variety, which in turn were combined into subclusters. Data of studies allow us to draw preliminary conclusions about the specificity of the genotypes of autochthonous yeasts found on the surface of white and red grape varieties.