Fruit growing
and viticulture of South Russia

Annotation

The main grape varieties used as table varieties and for winemaking belong to the species Vitis vinifera L., which has a high quality of berries, but rather low resistance to diseases and frost. One of the donors of valuable features f or the breeding of grape varieties is the species of Vitis amurensis Rupr. For example, some V. amurensis genotypes carry the downy mildew resistance genes of Rpv10 and Rpv12. The aim of the work is to study the genetic potential of resistance to downy mildew by DNA-marker analysis of some hybrid forms obtained using Amur grapevines. The research was carried out on the DNA of twenty-one grape genotypes, created by F.I. Shatilov using V. amurensis, and preserved in the collection of A.Y. Nemytov (Orenburg Region). DNA extraction was carried out by CTAB method from herbalized young shoots of grape plants. In the work for identification gene Rpv10 the closely linked DNA marker GF09-46 was used to identify the Rpv12 gene � markers UDV360, UDV343, flanking the locus of resistance. Separation of reaction products and analysis of PCR fragment size were carried out using an automatic genetic analyzer. As control genotypes in the study the grape varieties those carriers the genes of Kunlean (Rpv12) and Saperavi Severnyi (Rpv10) were included. In two forms under the conventional names �Muscat Severniy No. 1� and �Hybrid form No. 3� the presence of genes Rpv12 and Rpv10 were revealed, respectively. These genotypes have a number of positive characteristics that are promising in the breeding of table grape varieties and can be considered as the sources o f valuable traits.