Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia

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Kozhevnikov Evgeniy Anatolievich

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Articles in journal: (total 5)

pdf
696 Кб
12 с.
Genetic Resources, Cultivar, selection
Date posted: 16.05.2022
UDC: 634.84: 575.113.2
DOI: 10.30679/2219-5335-2022-3-75-85-96
Keywords: WILD-GROWING VINES, DNA MARKERS, POLYMORPHISM

Annotation

On the territory of Krasnodar region, grapevines have been cultivated since ancient times. However, the study of the gene pool of local wild grapes remains an open question. This study is devoted to the research of wild growing grapevines found in the area of Tonkiy cape of Gelendzhik city. The ecological-geographical and climatic conditions of this territory can be considered as a favorable place for the growth of wild grapevines. Seven samples of vines were found, having some similarities and differences in ecological, biological and morphological indicators. Most of the studied samples are dioecious plants – there are samples with male and female flower types. No inflorescences were found on the one specimen. The material for DNA analysis was selected from the found grape samples. DNA was isolated by CTAB method from herbarized apical leaves. Genotyping of wild-growing forms was performed by microsatellite loci: VVS2, VVMD5, VVMD7, VVMD27, VrZag62, VrZag79 which are used as a standard set for grape varieties DNA fingerprinting, as well as by loci UDV737 and CenGen6, which are linked to locus of resistance to downy mildew Rpv3 and powdery mildew Ren9, respectively. Target fragments, the size of which indicates the presence of resistance genes, were not detected by DNA markers UDV737 and CenGen6. The obtained DNA profiles on microsatellite loci showed genetic differences of the vines in the analyzed sample. On average, 5 types of alleles per locus were identified. Cluster analysis of DNA profiling data showed that one of the found vines differs from the rest most significantly, the remaining samples were distributed into two subclusters of two and four genotypes according to the results of the analysis.

How to cite
Ilnitskaya E., Makarkina M., Gorbunov I., Kotlyar V., Kozhevnikov E. MORPHOLOGICAL AND GENETIC CHARACTERISTICS OF WILD-GROWING VINES ON TONKIY CAPE TERRITORY OF GELENDZHIK CITY [Electronic resource] // Fruit growing and viticulture of South Russia. 2022. β„– 75(3). pp. 85–96. URL: http://journalkubansad.ru/pdf/22/03/07.pdf. DOI: 10.30679/2219-5335-2022-3-75-85-96 (request date: 28.03.2024).
pdf
631 Кб
11 с.
Phytosanitary condition of plants
Date posted: 20.01.2021
UDC: 634.8: 632.4: 575.174.015.3
DOI: 10.30679/2219-5335-2021-1-67-294-304
Keywords: GRAPEVINE, DOWNY MILDEW, PLASMOPARA VITICOLA, PATHOGEN GENERATIONS, GENETIC DIVERSITY, DNA MARKERS

Annotation

Downy mildew is a vine disease caused by the obligate heterothallic biotrophic endoparasite Plasmopara viticola. The area of distribution of this pathogen are vineyards all over the world, but the greatest losses are in the viticulture zones with a temperate continental and subtropical climate. In Krasnodar Territory, epiphytotic development of mildew occurs 6-7 times in 10 years. The first molecular genetic studies of the pathogen began at the end of the 20th century. The purpose of this work is to evaluate, based on DNA marker analysis, the polymorphism of the P. viticola population in two generations of the pathogen on grape plants growing in an isolated geographic point. The material for the study was the affected grape leaves taken from the vegetation plot of the FSBSI NCFSCHVW, from plants without chemical treatment. The material was taken in two time intervals – the end of July (the first generation of the pathogen – 4 samples) and the middle of August (the second generation – 6 samples). To study the diversity, highly polymorphic SSR-markers – GOB and PV144, were used. P. viticola DNA was isolated directly from infected leaves by the CTAB method. A total of 10 DNA samples of the pathogen were analyzed. The study was carried out by the classical method of polymerase chain reaction. The size of the target fragments of the PV144 and GOB loci was estimated using an ABI Prism 3130 automatic genetic analyzer by fragment analysis. The data obtained were analyzed using Gene Mapper 4.1 software. The highest degree of polymorphism was found for the GOB marker – 7 types of alleles and to a lesser extent PV144 – 4 types of alleles. It was found that the samples of the pathogen population collected during the first generation have significantly higher genetic polymorphism, in contrast to the samples collected during the second generation. Research in this direction continues.

How to cite
Kozhevnikov E., Makarkina M., Ilnitskaya E. STUDY OF PLASMOPARA VITICOLA POPULATION POLYMORPHISM IN DIFFERENT PATHOGEN GENERATIONS [Electronic resource] // Fruit growing and viticulture of South Russia. 2021. β„– 67(1). pp. 294–304. URL: http://journalkubansad.ru/pdf/21/01/20.pdf. DOI: 10.30679/2219-5335-2021-1-67-294-304 (request date: 28.03.2024).
pdf
404 Кб
13 с.
Genetic Resources, Cultivar, selection
Date posted: 20.11.2022
UDC: 634.8: 575.113.2
DOI: 10.30679/2219-5335-2022-6-78-195-207
Keywords: WILD-GROWING VINES, POWDERY MILDEW, REN1, DNA MARKERS, POLYMORPHISM

Annotation

The study of wild-growing vines allows to expand fundamental knowledge about the diversity of the grape gene pool, and wild vines are also studied in order to identify potential sources of resistance to stress factors of growing conditions. Powdery mildew resistance gene Ren1 is one of the few pathogen resistance genes identified in Vitis vinifera. Its presence has also been found in some vines of V. vinifera ssp. silvestris Gmel. The aim of this work was to study wild-growing vines found in the Krasnodar region with DNA markers of Ren1 powdery mildew resistance gene in order to search for potential donors of resistance. The study was conducted on 35 genotypes of wild growing vines, selected at several geographical points of the Krasnodar region along the coastline of the Black Sea and the Kuban River. Polymorphism of Ren1 locus was studied by PCR using cosegregated markers SC8-0071-014 and sc47-18, followed by assessment of the size of amplified fragments by capillary gel electrophoresis on a Nanofor 05 device and statistical processing in the GenAlEx 6.5 program. As a result, in the studied sample of genotypes, a rather high polymorphism was revealed for studied microsatellite loci (SC8-0071-014 – 18 types of alleles, sc47-18 – 8 alleles) – an average of 13 alleles per locus was determined. In the prevailing majority of genotypes, the heterozygous state was determined for the studied loci, which is reflected in the indicators of expected (He) and observed (Ho) heterozygosity: the observed values exceed the expected ones. The resistance alleles of Ren1 gene were not found in any of the studied samples of wild grapes.

How to cite
Ilnitskaya E., Makarkina M., Kozhevnikov E., Pankin M. POLYMORPHISM OF MICROSATELLITE LOCI LINKED TO THE REN1 GENE IN THE GENOTYPES OF WILD-GROWING VINES OF THE KUBAN REGION [Electronic resource] // Fruit growing and viticulture of South Russia. 2022. β„– 78(6). pp. 195–207. URL: http://journalkubansad.ru/pdf/22/06/12.pdf. DOI: 10.30679/2219-5335-2022-6-78-195-207 (request date: 28.03.2024).
pdf
335 Кб
10 с.
Genetic Resources, Cultivar, selection
Date posted: 21.01.2022
UDC: 634.8.091: 575.113
DOI: 10.30679/2219-5335-2022-1-73-124-133
Keywords: GRAPE, DNA-MARKERS, BREEDING, RESISTANCE TO POWDERY MILDEW

Annotation

Powdery mildew of grapes or oidium is one of the most common and economically significant diseases of grapes. The causative agent of the disease is biotrophic ascomycete Erysiphe necator. Powdery mildew is a global disease that reduces the yield and quality of grapes, while causing significant damage to viticulture around the world. Most cultivated grape varieties are susceptible to this pathogen, therefore, pesticide treatment is used to reduce the incidence of disease. Resistance to powdery mildew is mainly found in the genotypes of North American and Asian grape varieties. The search for donors of resistance to powdery mildew and the subsequent creation of new resistant and high-quality grape varieties is one of the main tasks of grape breeding. Cultivation of resistant varieties will reduce the use of pesticides, which in the future will make it possible to switch to more environmentally safe industrial viticulture. At present, a number of loci of resistance to powdery mildew (more than 10) have been identified, and DNA markers have been constructed that are suitable for identifying the allelic status of these genes. Among the identified loci of resistance to powdery mildew, Ren3 and Ren9 genes have a large contribution. The study involved elite technical forms of grapes of the NCFSCHVW breeding, the genotypes of which could potentially contain loci of resistance to powdery mildew (Tana 19, Tana 72, Tana 73, Tana 74, Tana 82, Tana 92). To identify the genes that cause genetic resistance to powdery mildew, specific codominant SSR markers GF15-42, ScORGF15-02 were used to identify the Ren3 gene, and SSR marker CenGen6 to identify the Ren9 gene. As a positive control, we used the DNA of the cultivar Seyve Villard 12-375, which has resistance alleles. The work was carried out by the PCR method, with the separation of reaction products using the method of capillary electrophoresis on an automatic genetic analyzer Nanofor 05. As a result of DNA marker analysis, it was determined that the genotype Tana 92 contain the Ren9 gene. The Ren3 and Ren9 genes has been identified in grapevine Tana 73 and Tana 74.

How to cite
Ilnitskaya E., Makarkina M., Kozina T., Kozhevnikov E., Pyata E. DNA MARKER DETECTION OF REN3 AND REN9 POWDERY MILDEW RESISTANCE GENES IN ELITE GRAPEVINES OF NCFSCHVW BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2022. β„– 73(1). pp. 124–133. URL: http://journalkubansad.ru/pdf/22/01/11.pdf. DOI: 10.30679/2219-5335-2022-1-73-124-133 (request date: 28.03.2024).
pdf
584 Кб
13 с.
Genetic Resources, Cultivar, selection
Date posted: 27.03.2023
UDC: 634.8: 575.113.2
DOI: 10.30679/2219-5335-2023-2-80-48-60
Keywords: GRAPE VARIETY, GENOTYPE, DNA MARKERS, DNA PASSPORT, ORIGIN CLARIFICATION

Annotation

The use of DNA markers, along with classical identification methods based on morphological assessment, makes it possible to more accurately determine the varietal affiliation of grape plants, as well as clarify the origin of genotypes. The purpose of this work is to perform genotyping of grape varieties bred by the Dagestan Breeding Experimental Station of Viticulture and Vegetable Growing (DBESV&V) using microsatellite markers (SSR) used for varietal identification and clarify the origin of varieties using DNA analysis data. The objects of the study were six table grape varieties – Vezne, Dolchatyi, Zhemchuzhina Yuga, Zarya Derbenta, Leki and Muscat Derbenskiy. The DNA of the studied varieties was extracted by the CTAB method from herbarized plant material. Genotyping was carried out using a standard set of SSR markers: VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79 by PCR followed by analysis of the results on an ABI Prism 3130 genetic analyzer, the obtained data were processed using the GeneMapper v 4.1 program and aligned with reference variety Pinot noir. Pedigrees of varieties were analyzed based on the principle of codominant inheritance of alleles from parents to offspring. DNA profiles of parental forms (Csaba gyoengye, Muscat of Alexandria, Muscat Hamburg, Kirovabadskiy stolovyi) of the studied varieties were taken from the international database of grape varieties VIVC (Vitis International Variety Catalogue), and we also used the previously developed DNA passport of Agadai variety. As a result of the study, DNA-passports of 6 varieties of DBESV&V breeding were developed. DNA analysis confirmed origin of grape varieties Vezne, Dolchatyi, Zhemchuzhina Yuga, Zarya Derbenta, Leki and Muskat Derbentskiy from the declared parental forms.

How to cite
Makarkina M., Ilnitskaya E., Kozina T., Kozhevnikov E., Kazahmedov R. DNA-IDENTIFICATION AND ANALYSIS OF PEDIGREE OF GRAPE VARIETIES DBESV&V BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2023. β„– 80(2). pp. 48–60. URL: http://journalkubansad.ru/pdf/23/02/04.pdf. DOI: 10.30679/2219-5335-2023-2-80-48-60 (request date: 28.03.2024).