Fruit growing
and viticulture of South Russia

Annotation

In the protection of fruit crops from a complex of pathogens, one of the current tasks is to prevent the development of root rot, among the causes of which are the activity of soil micromycetes Phytophthora sp. Selective media play a key role both in identification and subsequent storage in the laboratory, and in the study of morphological and cultural traits, sporulation ability, as well as to obtain a certain amount of inoculum (for example, for in vivo studies) and mycelium (for DNA isolation). Obtaining a pure culture of pathogenic micromycete with certain morphological and cultural characteristics, which may be important for diagnosis by both traditional and modern methods, determines the relevance of studying the influence of various media on these parameters of the pathogenic fungus Phytophthora sp. The studies were carried out by methods of laboratory and comparative analyses. The growth of the pathogen strain of apple root rot Phytophthora sp. on nine nutrient media was studied. The colony growth rate and morphological and cultural characteristics were evaluated. As a result of the research, the strain has been found to vary not only the growth rate of the colony, but also the cultural characteristics depending on the nutrient medium. Of the nine nutrient media, three universal ones were selected that are most suitable for cultivation and identification, namely, tomato, potato-glucose and must agars. They meet the following criteria: ensuring the maximum degree of sporulation, rapid growth and development of the mycelium of the fungus, ease of preparation. It was also noted that Leroux medium can be used to obtain a large amount of inoculum, since it will produce more spores when planted in a lawn than on other media.