Fruit growing
and viticulture of South Russia

Annotation

The characteristic �strawberry� aroma of strawberry fruit is due to the presence of methyl anthranilate (methyl ester of anthranilic acid). The present study shows the results of the marker-assisted screening of promising strawberry selected forms created in the �I.V. Michurin FSC�, according to the FanAAMT gene controlling biosynthesis of methyl anthranilate. The biological objects of the study were strawberry selected hybrid forms 298-19-9-43 (FB2 F. orientalis, F. moschata, F. × ananassa), 26-5, 928-12, 913-102, 913-157 (FB3 F. orientalis, F. moschata, F. × ananassa), 932-29 (F. virginiana subsp. platypetala × Feyyerverk), 56-5, 56-18, 56-20 (Gigantella Maxim × Privlekatelnaya), 65-26 (Olimpiyskaya nadezhda × Bylinnaya), 61-22 (Bylinnaya × Olimpiyskaya nadezhda), 75-80, 75-68 (Tokado × Elianny), 28-11, 28-36 (Lakomaya× Maryshka), 21-14 (Urozhaynaya CGL × Rubinovyy kulon), and 72-67 (Privlekatelnaya × Bylinnaya). Total genomic strawberry DNA was extracted according to the modified CTAB method. The FanAAMT gene was identified with primers FanAAMT for/rev. As a result of the studies, the genetic sources of the functional allele of the FanAAMT gene were identified � strawberry selected forms 26-5 (Rubinovyy kulon× (([(F. orientalis Los. × Cavalier) × F. moschata Duch.] × Redcoat) × Senga Sengana)) and 928-12 ((([(F. orientalis Los. × Cavalier) × F. moschata Duch.] × Redcoat) × Senga Sengana) × Privlekatelnaya). It was found that the parental strawberry forms � wild species F. virginiana subsp. platypetala strawberry varieties Bylinnaya, Privlekatelnaya, Rubinovyy kulon, Feyyerverk are characterized by a heterozygous combination of alleles of the FanAAMT gene. The strawberry selected forms 26-5 and 928-12 are also characterized by a heterozygous genotype for the FaOMT mesifurane biosynthesis gene, which allows them to be used as complex sources of fruit aromatic complex genes.