Fruit growing
and viticulture of South Russia

Annotation

Studies were conducted in 2019 in the North Caucasus FSAC. The aim of the research is to determine the effectiveness of the use of various concentrations of Benzichol and Etichol preparations in a solid nutrient medium to accelerate the growth and development of in vitro improved micro-cuttings of grapes during clonal propagation. The results of studies on the influence of new synthetic preparations, that offering phytoregulatory, stress-protective, auxin, retardant, and antiviral activity, used in very low doses, which stimulate and accelerate the growth and development of in vitro improved micro-cuttings of grapes are presented. The middle-early Alminskiy variety was used in the experiment. Studies have shown that the addition of phytoregulators and stress-protectors Benzichol and Etihol to the solid nutrient medium according to Murashige and Skoog, for growing in vitro improved micro-cuttings of middle-early Alminskiy variety, at studied concentrations (1×10-7 ; 1×10-9 and 1×10-11 M) did not contribute to the acceleration of the root formation and growth in improved grapes micro-cuttings. At the same time, the use of Benzichol at concentrations of 1×10-7 and 1×10-9 , on the contrary, led to a significant slowdown in root formation in plants. The addition of Benzichol and Etihol preparations to the nutrient medium at a minimum concentration of 1×10-11M had a stimulating effect on the formation of internodes, after 20 days the average number of internodes on 1 plant increased up 0.5-0.7 pcs relative to the control. With the further growth of plants after 30 days, the trend noted above remained, although the difference with control decreased to 0.3 pcs. It indicates the possibility of using these preparations in order to increase the production of high-quality homogeneous virus-free planting material, and later to reproduce healthy young plants, which are necessary for establishing of vineyards.