Fruit growing
and viticulture of South Russia

Annotation

Adaptation of plants to non-sterile conditions is the last stage of reproduction in vitro and should be gradual in order to avoid the ruin of plants due to a sudden change in relative humidity, light and temperature. Rooting ex vitro allows to simplify this stage and simultaneously to obtain the plants adapted to natural conditions. The purpose of these study is to determine the optimal conditions for rhizogenesis and adaptation of blueberry micro shoots. Research was carried out in the Department of Biotechnology of the Republican Unitary Enterprise "Fruit Growing Institute" (Belarus). The material for the study was the regenerating plants of Northblue blueberry (V. angustifolium Ait. × V. corymbosum L.). As a result of carried out study, it was found that in vitro conditions the proportion of rooted regenerating plants of Northblue blueberry after four weeks of cultivation was 0-36.7 %, after eight week � the number of rooted regenerants increased to 63.3%. At the same time, a high frequency (100%) of callus formation was a negative phenomenon of blue berry rooting in vitro on media with IBA and IAA. On medium without auxin after 4 weeks, callus was absent, after 8 weeks � the frequency of callus formation was 36.7 %. After eight weeks of cultivation, the highest increasing of shoot's growth regenerating Northblue plants was on a nutrient medium with 0.3 mg / l IBA. The best length of the roots was obtained on nutrient medium with 1 mg / l IAA. Study have shown that the type of substrate affects growth (p