Fruit growing
and viticulture of South Russia
Babakina Mariya
Articles in journal: (total 6)
When developing new biotechnological solutions for the agro-industrial complex, one of the key problems is the preservation of the viability of microorganisms producers of biologically active substances. Drying is the main method for stabilizing microorganisms. During the drying process, cells are exposed to damaging stress factors that reduce their viability. Projective environments protect cells from stressors. Microorganisms belonging to unstable species, when dried without the use of protective media, are subject to significant changes, accompanied not only by the loss of viability, but also by the destruction of their cellular structure. In this regard, in the course of the study, the optimal drying mode was selected, which provides for intensive moisture removal at the very beginning of drying and warming up to a temperature not higher than + 32-35 ºC throughout the entire drying time. It should be noted that the entire drying process should take no more than 30 minutes, since when drying microorganisms by the second mode with a drying duration of 37 minutes, the number of dead cells was slightly higher than with drying by the first mode. Also, during the study, a protective environment was selected, consisting of 1 % gelatin, 5 % sodium glutamate and 5 % sucrose, which showed the best results as protection of the cells of the consortium under study from the damaging drying factor and subsequent rehydration. Also, when using this protective environment, the moisture content of the consortium under study was the lowest among the selected drying options.