Fruit growing
and viticulture of South Russia

Annotation

The paper presents an assessment of the effectiveness of clonal propagation of promising clonal rootstocks for cherry and sweet cherry. The demand in clonal rootstocks for small-stone crops is due to the intensification of their production. Clonal rootstocks of FSBSI NCFSCHVW breeding of the AI series meet the requirements for rootstocks: they provide restrained plant growth, a compact crown of trees, and are adapted to the edaphoclimatic conditions of the region. AI rootstock explants are best introduced during the period of active shoot growth from April 3rd to early June for propagation of rootstocks in in vitro culture. In this period, the regeneration of explants is the highest and, depending on the genotype, varies from 67.6 to 75.6 % in AI 1, from 7.2 to 83.4% in AI 11 and from 66.7 to 75, 85% in AI 70. At the stage of multiplication in the presence of 6-BAP at a concentration of 1 mg/l, all rootstocks develop well on Murashige-Skoog (MS) and DKW nutrient media. The largest number of shoots already after the first passage was noted on the DKW medium with the addition of Fe-EDDHA � 6.0-8.4 units/exp, with the addition of iron chelate Fe-EDTA, depending on the doubling, it is 4.6 up to 6.3 shoots per explant. In the control variant, 3.9 to 4.9 shoots are formed from one explant on MS medium with Fe-EDTA. In general, AI 11 and AI 70 rootstocks have a higher reproduction potential in all variants. Dispersion analysis showed a reliable influence of the factors �Nutrient medium� and �Form of iron chelate� on the reproduction coefficient for all studied rootstocks. The interaction both factors was observed only in AI 70 rootstocks. At the stage of micropropagation of AI series rootstocks, it is recommended to use DKW nutrient medium with iron chelate form Fe-EDDHA (100 mg/l) and 6-BAP 1.0 mg/l.