Fruit growing
and viticulture of South Russia
Ilnitskaya Elena
Articles in journal: (total 42)
The aim of the present work was the DNA fingerprinting of a number of promising grapevine cultivars of NCRRIH&V and AZESV&W breeding, based on the analysis of polymorphism of microsatellite loci. The results of the study can be used for identifying of the studied cultivars and for control purebred of parent vines plantations and planting material of domestic cultivars. Domestic breeding cultivars, promising to improve the industrial assortment of grapes in the Southern Russia were studied on the molecular genetic level. The article presents the results of a DNA-fingerprinting of six domestic cultivars of grapes: Barchatny, Vladimir, Dmitry, Dostoyny, Kurchanskiy, Krasnostop AZOS. Analysis of cultivars genotypes carried out on the mixture of DNA samples of 7-10 typical plants of each cultivar. DNA profiles were obtained by microsatellite loci MD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79, these markers are recommended as the basic ones for Vitis vinifera L. genotyping. Microsatellites (SSR-simple sequence repeats) - tandem repeats of simple sequences in the DNA structure are the most often used as DNA markers for studying of the grapes genotypes. The main method used in the work - polymerase chain reaction with the separation of the reaction products using an automated genetic analyzer ABI Prism 3130. The DNA of Chardonnay and Cabernet Sauvignon were used as the control, allelic compositions of studied SSR-loci are known for these cultivars. Cultivars genotypes showed the different alleles combinations on studied loci. Thus, the minimum recommended set of microsatellite markers is sufficient to differentiate of studied genotypes. The article also provides a brief description of the studied cultivars and their main distinguishing positive agric and biological characteristics.
Downy mildew is the one of the most prevalent diseases of the grapevine. An effective way to control the spread of disease is cultivation of resistant varieties. The European grape V. vinifera is not resistant to mildew practically. Genotypes, carrying the resistance genes, belong to the grape varieties of North America and Asia. So breeding of resistant grape varieties is based on interspecies hybridization. Modern methods of genetics and molecular biology expand the possibilities of ongoing research in this field. Today it is identified about 20 major and minor of resistance loci to P. viticola. Rpv10 and Rpv3 are major loci of resistance. Rpv10, inherited from V. amurensis was first determined F. Schwander et al. It was identified and a DNA-marker GF09-46, closely linked with this locus. Rpv3 gene was first found in the Bianca grapes a complex interspecies hybrids of grapes. It was later determined that the Rpv3 gene has seven haplotypes, and their identification is possible with microsatellite markers UDV305, UDV737. We analyzed the genotypes of selected, elite forms and new inter-species grape varieties of NCFSCHVW breeding by the PCR method using DNA markers linked to the mildew resistance Rpv10 and Rpv3 genes. The separation of the reaction products is carried out by capillary electrophoresis using an automated genetic analyzer ABI Prism 3130. The analyzes were carried out in accordance with the potential possibility of identifying the target alleles according to the pedigree of the studied samples. The Rpv3 gene is defined in six hybrid genotypes, Rpv10 gene is defined in one grape hybrid.
Polymorphism of 10 microsatellite loci of Cabernet Sauvignon, Saperavi grapes and their certified clones was studied. An analysis of previously identified by clonal selection grapes protoclones is conducted with perspective for clonal polymorphism identification SSR-markers. 7 protoclones of Cabernet Sauvignon and 2 protoclones of Saperavi with differences in the studied loci from original cultivares are allocated.
The description of new grapes varieties of the NCRRIH&V breeding with high resistance to diseases and frost for qualitative red wine making is presented in the article.