Fruit growing
and viticulture of South Russia

Annotation

The method of embryo culture has more and more wide application in the interspecific hybridization of fruit plants. For its improvement the hormonal regulation of growth and development of embryos in vitro is researched and the optimal age of embryos injected into culture is defined. The main objective of the presented work is to receive the viable plants from puny seeds and not sprout embryos of hybrids of sweet cherry and cherry in the course of their cultivation in vitro and to allocate the effective growth regulators and to establish the optimal terms of embryos input in the culture. The results of research on cultivation in vitro of sweet cherry and cherry of 16 combinations of crossing are presented. The regenerants of hybrid embryos of cherry and sweet cherry in vitro culture from 16 combinations of crossings are received. For embryos culture of sweet cherry and cherry the best modification of Murashige-Skoog environment with 79 % level of embryos regeneration is allocated. The most effective concentration of gibberellic acid in the environment for cultivation of cherry and sweet cherry is revealed. That is 10 mg of gibberellic acid per 1 liter of the Murashige-Skoog environment, for all that the embryo size should be maximized.