Fruit growing
and viticulture of South Russia

Annotation

Biotechnological methods are used widely in the world for prolonged conservation of plants collections for further their use in the breeding and production of virus-free planting material and for conservation of gene pool and biodiversity of plants. One of the methodological approaches to the deposition of the plants is the maintenance of biological objects under the conditions of slow metabolism. It is known that in a nutrient medium, for the growth and preservation of plant material in vitro, the various organic substances having a high osmotic activity are added. The researchers note that increased concentration of sucrose (4-5%) in the nutrient medium inhibited the growth of cells without causing tox-ic effects, and therefore can be used to maintain cultures in a state of rest over a long period of time. The aim of our study was to identify the effect of sucrose concentrations the growth processes of grapes mericlones for prolonged storage of plants in the collection in vitro. When cultivated in vitro, we used the sucrose concentration from 10 to 90 g/l, and evaluated the reaction of different grapes varieties to higher concentrations. In our experiment we carried out the cultivation without replanting of 12 grapes varieties for 9 months at a sucrose concentration of 70 g/l while the plants were viable. It was proved that by reducing of sucrose content in the nutrient medium from 70,0 g/l to 60.0 g/l the duration of deposit of different grapes varieties elongated to 1 year. By carried out research we have been found the deceleration of growth processes and the possibility of elongation of cultivation without replanting to create a collection of grapes in vitro.