Fruit growing
and viticulture of South Russia

Annotation

The study and generalization of world experience in research on the development of technologies to reduce losses, stabilize the quality and maximize the preservation of biologically active substances of crop products during storage, made it possible to conclude that research on the methodology for the application of electromagnetic fields of extremely low frequencies (EMF ELF) is relevant. to control the development of pathogenic microflora, leading to the development of diseases and, as a consequence, the loss of crop production. During the study, the composition of the microflora of strawberries was studied, it was found that the amount of mesophilic aerobic and optionally anaerobic microorganisms (MAaOAM) on the berries of the strawberry variety �Marmalade�, the harvest of 2019, ranged from 5000 CFU/g to 12000 CFU/g. MAaOAM are mainly represented by spore microorganisms, enterobacteria and yeast. The number of molds is from 110 CFU/g to 1400 CFU/g. The influence of EMF treatment by electromagnetic fields on the pathogens of strawberry fungal nature was investigated. In the samples treated with EMF ELF, in comparison with the control sample, the number of microorganisms after 14 days of storage was lower. The regularities of the influence of the EMF ELF processing parameters on the development of pathogenic microorganisms of strawberries in experiments and in vivo were revealed. It was established that the processing of strawberries of the variety �Marmelada� using ELF EMF with parameters: frequency � 30 Hz, current strength � 10. A, processing time � 30 minutes has the maximum inhibitory effect on the development of phytopathogenic microorganisms Botrytis cinerea and Rhizopus nigricans.

Annotation

The problem of storing fruits and vegetables and preserving their sensory and nutritional qualities is actual nowadays. It is extremely important to find such a technology for preserving fresh fruits and vegetables and with the use of which all the components of their chemical composition remain at a high level for the longest time in terms of their content and do not undergo significant changes, biological activity and safety. To ensure stabilization of commercial quality, maximum preservation of biologically active substances and reduction of losses during storage of berry raw materials, various technologies are used � such as controlled gas environment, treatment with chemical reagents, microbiological preparations, the use of various physical factors. A comparative analysis of the growth dynamics of the populations of the fungal pathogens Botrytis cinerea and Rhizopus stolonifer was carried out depending on the parameters of their treatment with ELF EMF. The regularities of the influence of the ELF EMF processing parameters on the development of pathogenic microorganisms of strawberries in experiments and in vitro were revealed. It was established that in the experimental sample Botrytis cinerea treated with ELF EMF with parameters of 30 Hz, 10 A, 30 minutes, the number of colonies grown after 7 days was 1.8 × 102 CFU, which is 27.5% lower compared to the references. In the experimental sample of Rhizopus stolonifer treated with ELF EMF with parameters of 30 Hz, 10 A, 30 minutes, the number of colonies grown after 7 days was 2 × 102 CFU, which is 26.2% lower compared to the references. An analysis of the data obtained in vitro experiments allows us to conclude that the variant of processing ELF EMF with parameters of 30 Hz, 10 A, 30 minutes most effectively affects the inhibition of the growth rate of the fungal pathogens Botrytis cinerea and Rhizopus stolonifer, which cause diseases of strawberry berries.

Annotation

The use of fresh fruits and vegetables in food is an integral part of a balanced human diet. The current trend towards a healthy lifestyle and taking care of one's health creates a high consumer demand for high-quality fresh fruits and vegetables. In recent years, in connection with global environmental problems, such as heavy air and water pollution, the agricultural industry has been faced with big problems of growing and post-harvest conservation of fruits and vegetables. A pressing problem is the storage of fruits and vegetables and the preservation of their sensory and nutritional qualities. It is extremely important to find such a technology for preserving fresh fruits and vegetables, when used, all components of their chemical composition as long as possible remain at a high level in content and do not undergo significant changes in quality indicators, biological activity and safety. The development of new technologies for the preservation of quality and the extension of the shelf life of fresh agricultural products is particularly important due to global environmental changes. The article discusses modern physical, chemical and biological studies that describe new ways to extend the shelf life of fresh fruits and vegetables.

Annotation

Cabbage vegetables are distinguished by a number of features of the course of physiological and biochemical processes due to high humidity, which increases the intensity of metabolism in cells and tissues. This fact makes it difficult to organize the storage of such primary produce: the quality is rapidly decreasing, losses are increasing, the value of which is influenced by such parameters as relative air humidity, temperature, degree of aeration, variety, degree of maturity of primary produce, presence of mechanical and other damage, phytopathogens contamination. In this regard, it is relevant to study the regularities of the effect of processing plant primary produce (white cabbage) with biological preparations based on domestically produced reagents on reducing losses, stabilizing quality and preserving biologically active substances. In experiments in vitro it was found that in relation to the phytopathogen Ps. maculicola, the biological product Vitaplan did not give the effect of suppressing growth, and the biological product Bactofit caused a slight delay in the growth of the phytopathogen: 0.5-1 mm. The effectiveness of the bactericidal action of natamycin on Rh. solani and Ps. maculicola is more than 3 times higher at a concentration of 0.1 g/l. The bactericidal effect of the product with a concentration of 0.5 g/l is more than 2 times more effective than a concentration of 0.1 g/l on exposure to S. sclerotiorum and B. cinerea. In an in vivo study, the development of Bacillus subtilis was not observed in samples with introduced biological products. In samples with introduced phytopathogens S. sclerotiorum, B. cinerea, Ps. maculicola, after 21 days of storage, the affected area increased threefold. With the joint introduction of phytopathogens and biological products, both Vitaplan and Baktofit had a restraining effect on the development of all three phytopathogens. No development of phytopathogens was observed during inoculation of white cabbage heads with phytopathogens followed by the introduction of two different concentrations of natamycin (0.05% and 0.1%).

Annotation

The losses of crop production during storage depend on variety characteristics, the system of protection against vermins and diseases, terms and methods of harvesting, commodity processing and storage preparation methods and are associated with physiological, biochemical and microbiological processes occurring during storage. In connection with this, the current direction of research is the development, modification and improvement of methods for managing the biochemical and microbiological processes of long-term storage of crop production. Research has been carried out in the field of biological control of phytopathogens � causative agents of carrot diseases using strains of Bacillus subtilis. It is shown that the most active in relation to the test set of pathogens characteristic for carrots in vitro experiments were strains of Bacillus subtilis IPM 215 and VKM B-2605D. The dynamics of populations of phytopathogens and antagonist strains in the sections of carrot roots was studied. It is revealed that the rate of growth of the Bacillus subtilis population in the first stage of storage exceeds the growth rate of the populations Alternaria radicina and Erwinia carotovora. After three days of storage, the growth dynamics of Bacillus subtilis decreases, which indicates the expediency of processing root crops with Bacillus subtilis preparations, which, due to more intensive growth, creates the competition in the struggle for nutrients during the first stage of storage. In addition, the products of the life of Bacillus subtilis are effective inhibitors of phytopathogens. As a result of carried out research it is established that the treatment of root crops of Bacillus subtilis influences the degree of development of diseases caused by Alternaria radicina and Erwinia carotovora that allows to consider the studied strains of Bacillus subtilis as promising for the biological control of diseases caused by phytopathogenic microorganisms during storage of root crops of carrots.