Fruit growing
and viticulture of South Russia

Annotation

Research was conducted to identify and study the technological characteristics of autochthonous strains of wine yeast isolated from vineyards in the Krasnodar region. From natural populations, 90 yeast strains were isolated from the surface of grapes of the following varieties: Petit Mansan, Merlot, Krasnostop Anapskiy. Generic identification of the studied strains was carried out. The proportion of Saccharomycetes varied over a wide range. Analysis of 90 yeast strains made it possible to isolate 40 representatives of the genus Saccharomyces. Species identification was carried out using molecular genetic methods. A working sample of 40 strains of Saccharomyces cerevisiae was obtained. Yeast screening for the ability to ferment sugars made it possible to isolate 13 yeast strains. Strains TK20-2 and LM19-19 are noted as weak fermenters with the formation of non-fermenters. An analysis of the ability of yeast to synthesize hydrogen sulfide showed that 21 strains produce hydrogen sulfide in an insignificant amount. In the course of studying the main technological properties, 4 strains were isolated, with the use of which white wine materials were prepared from the Onitskanskiy grape variety. In wine materials produced using local strains, a variation in the concentrations of organic acids, amino acids and volatile aroma-forming components was noted. The conducted tasting of the studied samples showed that 3 out of 4 wine materials were characterized by high quality. The sample produced using strain LM19-3 had the highest tasting score. The use of strains LP19-1, TK20-11 and LM19-3 ensures the production of high-quality wine materials with high organoleptic characteristics.

Annotation

In recent years, many wineries have chosen to avoid using commercial S. cerevisiae strains as fermentation starters, and preferred the use of autochthonous selected strains as an oenological strategy that allows combining the industrial advantages of inoculated fermentation with the authenticity and typicality of spontaneous fermentation by native S. cerevisiae strains. One of the important tasks in the search for autochthonous strains of S. cerevisiae is the assessment of the genetic identity of strains and their genetic diversity. The interdelta genotyping method is successfully used for this purpose. The paper presents the results of interdelta genotyping of 49 autochthonous S. cerevisiae strains isolated on white and red grape varieties on the territory of the Krasnodar region, using three primer pairs δ1+ δ2, δ12+ δ2, δ12+ δ21. It was found that when primer pairs δ12+ δ2 and δ12+ δ21 were used, 14 different patterns were formed in the samples. When using the primer pair δ1+ δ2 � 11 patterns. With the primer pair δ12+ δ21, 5 and 6 groups of genetic similarity were formed in the studied samples, which is higher than when using other types of δ pairs. According to the dendrogram obtained from the results of the interdelta analysis and constructed using the UPGMA method, the strains were divided into 2 main groups, and the first group included only yeasts isolated on the Dostoynyi grape variety (19 out of 28 strains). The second group included 9 strains isolated on the Dostoynyi grape variety and all strains isolated on the Chardonnay grape variety, which in turn were combined into subclusters. Data of studies allow us to draw preliminary conclusions about the specificity of the genotypes of autochthonous yeasts found on the surface of white and red grape varieties.

Annotation

The issue of obtaining planting material for fruit crops, free from pathogens, including viruses and phytoplasma, is of high relevance at the present time. The use of modern methods of biotechnology and molecular genetics is an important element in the production of improved planting material, since it allows not only to obtain plants in the culture of meristems in vitro and to produce their microclonal propagation, but also allows control the presence of viral and phytoplasmic pathogens, the identification of which by external symptoms can be difficult due to the presence of a latent form of disease. The article presents the results of the implementation of complex studies on microclonal reproduction of various crops of fruit crops, as well as an analysis of modern scientific literature on this topic. In addition, the results of molecular genetic studies in the part of the analysis of the genetic stability of plants obtained in vitro and the identification of viruses are presented. It was noted that at all stages of micropropagation, including the use of a culture of meristems, it is often necessary to develop variety-specific protocols. Optimal protocols for sterilization of explants of pome and stone fruit cultures using disinfecting tablets "OKA-TAB" and antimicrobial preparation �BIO-PAK� have been developed. The dependence of the intensity of the release of phenolic compounds by explants and their further necrotization upon introduction into culture in vitro on the phases of development and the age of donor plants was revealed. The optimal concentrations of nutrient media components, as well as phytohormones, have been established for a number of varieties and rootstocks of pome and stone fruit crops of domestic breeding. As part of the analysis of the genetic stability of plants obtained under in vitro conditions, ISSR and IRAP DNA markers have been identified that are promising for use for these purposes on apple stocks (ISSR: UBC 811, UBC 841 and UBC 843; IRAP: Cass 1 and �ass 2). When analyzing the genetic stability of the rootstocks of large-stone cultures using ISSR DNA markers, the identity of the DNA profiles was revealed, which testifies to the preservation of genetic stability for this rootstock using the developed micropropagation protocols. Based on the use of a complex of biotechnological and molecular genetic methods, genetically homogeneous rootstocks of stone fruit cultures, free from the virus pox disease of plum, were obtained. These plants, after testing for other viruses of stone crops and in the absence of such, will be transferred to the category of �candidates for initial� and, after retesting, can be transferred to the category of �initial plants� and serve as a basis for further mass production of virus-free rootstocks.

Annotation

The modern wine industry for the production of wines uses imported cultures of active dry yeast, which often require adaptation to local production conditions. According to numerous data, the best wines can be produced only with the use of local yeast strains, adapted to the conditions of a particular locality and the chemical composition of berries, in particular, the acidity of the juice, the pH value, in connection with which the fermentation of the must proceeds more actively, and the resulting product has a higher quality. At the moment, there are a number of examples of successful implementation of projects to search for promising commercial strains of wine yeast among wild populations to obtain terroir-type wines with high taste characteristics. The work presents a morphological and cultural assessment of yeast strains isolated on 17 grape varieties at 6 points of selection (AF �Yuzhnaya�, farms, private farmstead) of Krasnodar region. A total of 510 yeast monocultures were isolated. An elective test and ITS analysis of some of the strains were carried out. According to the morphological assessment of yeast isolates, the cells of the Saccharomyces yeast were round, oval, or oval-shaped. The cells of the yeast of non-saccharomyces were elliptical, lemon-shaped, rod-shaped. It was found that the proportion of saccharomycetes varied depending on the grape variety and the place of its growth. Samples in which Saccharomyces were not found were identified. Restriction analysis of yeast isolates of the genus Saccharomyces was carried out for 153 strains isolated in the village Tamanskiy, AF �Yuzhnaya�: Cabernet Sauvignon, Krasnostop Anapskiy, Pervenets Magaracha, Zweigelt; and in Novorossiysk, �Usad�ba Semigorye�: Sauvignon Blanc, Kristall, Cabernet Fran. It was found that all studied samples of the genus Saccharomyces belong to the species S. cerevisiae / S. paradoxus.

Annotation

The publication presents the results of a study aimed at assessment of introduced ultra � early-maturing forms of walnut according to such traits as fruit ripening time, plant habit, f ruit-bearing type, ratio of fruit setting from lateral and apical buds, number of fruits in nut cluster, fruit characteristics, including a number of biochemical parameters. Based on the results of the observation, it was concluded that there is a trait of restrained growth in all the studied samples. At the same time, the level of compactness of crown branching had insignificant differences. The degree of crown compactness in all samples, with the exception of sample 16-SI-6, is comparable to the Dachnyi variety. The indicated sample had a more compact crown close to spur. It was found that all the studied samples have a lateral type of fruit-bearing (from 50 to 8 0% of the fruits are formed on lateral shoots). It has been established that the formation of nut clusters with two or three fruits, which, along with lateral fruit-bearing, contributes to an increase in productivity potential. According to these features, all studied forms are sources of traits. Taking into account such characteristics as the yield of the kernel, color and its extractability, the following most promising samples can be determined: 16-SI-5, 16-SI-6, 16-SI-10, 16-T-1, 16-T-2. They are sources of a set of selection-valuable traits and can be used in breeding. Based on the data of biochemical analysis, a sample 16-SI-2was identified, which has an increased content of biologically active substances, as well as fats, which may be the basis for its use in breeding according to these traits. The forms 16-SI-6 and 16-T-1 can be further considered as candidates for the varieties most promising for use in the private sector.