Fruit growing
and viticulture of South Russia

Annotation

Bacterial cancer of grapes is the most dangerous and wide spread disease of grapes in a world. In the Krasnodar Region now the death of grapes plant-ings from this disease has increased in 2-2,5 times. The causative agent of a disease often extends in new areas with landing material without symptoms of disease; therefore the diagnosis of bacterial cancer is an important task of wine growing branch. This article is devoted to this question. For research we have taken the tumorous outgrowths of bacterial cancer found on grapes plants of various ecological-geographical origin (Italy, France, Serbia, Russia) on vineyards of Krasnodar Region. In total 22 grapes samples are analysed. DNA from tumors is defined using the modified CTAB method. For research of isolates of Agrobacterium we used two methods of the polymerase chain reaction (PCR) - classical PCR and PCR in real time (PCR-RV). By PCR-RV method with use the test-system to pehA gene the presence of DNA in all analyzed samples of the studied grapes varieties is detected. Selective amplification of six DNA samples by PCR classical method with the use of test-system to virF gene has revealed the target fragments in all studied grapes samples. In these samples of the tumorous gall outgrowths found on grapes plants in the territory of Krasnodar Region the agrobacterium of A. vitis type is revealed as the causative agent of bacterial cancer of grapes. Accoding to the results of the carried out research we came to a conclusion that the use of methods of molecular and genetic identification allows us to give a diagnosis quickly and precisely of the causative agent of bacterial cancer disease for grapes plants.