Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia



Ilnitskaya Elena

Federal State Budget Scientific Organization "North Caucasian of Horticulture and Viticulture"

Candidate of Biological Sciences

Articles in journal: (total 18)

pdf
636 Кб
10 с.
Genetic Resources, Cultivar, selection
Date posted: 14.05.2018
UDC: 634.8: 631.52
DOI: 10.30679/2219-5335-2018-3-51-50-59
Keywords: DATABASE, GENE POOL, GRAPES, VARIETY, DNA-CERTIFICATION, AMPELOGRAPHIC COLLECTION

Annotation

At the present stage of studying the genetic grape resources, the DNA profiles addit the traditional ampelographic descriptions, agrobiological and economic characteristics of varieties and serve as a basis for reliable identification of genotypes, therefore, in order to solve the priority tasks of vine breeding, it is necessary to mobilize and conserve the genetic resources, with the creation of an electronic database. Ampelographic collections are very important in the preservation and use of the gene grape pool, in these collections the work is constantly carried out to introduce varieties, to study of their agrobiological, phonological and economic features, to identify the promising ones from them, and to select the donors for use in breeding work in order to obtain the new competitive varieties. In the Anapa ampelographic collection with the participation of the laboratory of variety study and preservation of the grape gene pool of NCFSCHVW the DNA-certification of grape varieties is carried out, a collection database has been created. Among the features of the software platform there is the search for samples in the database for a number of parameters, displaying a common list of samples and displaying the detailed information for each variety separately. Services for calculating and analyzing of agro-biological and phenological observations data, analyzing the safety of buds after wintering of plants according to bushplaces in an ampelocollection have been realized. The DNA certification was carried out for the grape varieties of AZESVW breeding the branch of the FSBSI NCFSCHVW (10 varieties), the varieties of the FSBSI NCFSCHVW (9 varieties) and 4 well-known European varieties, which will be integrated into a united database. The obtained DNA-passports of varieties in practice can be effectively applied to determine the clean varieties in the planting material and in the grapes orchards, to define the parental forms of the sample, as well as in the disputed questions of the variety authorship.

How to cite
Lukyanov A., Bolsakov V., Ilnitskaya E. CREATION OF DATABASE AND DNA-SERTIFICATION OF VARIETIES OF ANAPIC AMPELOGRAPHIC COLLECTION [Electronic resource] // Fruit growing and viticulture of South Russia. 2018. № 51(3). pp. 50–59. URL: http://journalkubansad.ru/pdf/18/03/05.pdf. DOI: 10.30679/2219-5335-2018-3-51-50-59 (request date: 16.12.2018).
pdf
696 Кб
10 с.
Genetic Resources, Cultivar, selection
Date posted: 14.05.2018
UDC: 634.527: 634.84: 634.8.091-93
DOI: 10.30679/2219-5335-2018-3-51-79-88
Keywords: GRAPES, GENOTYPE, PHENOTYPE, DNA-PASSPORT, INDIGENOUS VARIETIES

Annotation

The purpose of this work is to determine the inheritance of genotypic traits and phenotypic features in the posterity of indigenous Daghestan grape varieties of Gimra and Asyl Kara, using as the example a new search was carried out with grape varieties from the Ampelographic collection of the Daghestan Selection Testing Station of Viticulture and Horticulture: as Gimra (Tavalinsky Ranniy), Asyl Kara (Kizlyar Cherny) and a new variety of Fioletta (Gimra Asyl Kara). The culture of the grapes is on own roots and with use of irrigation. In the process of research the next methods were used: field study of phenological, morphological and biological features and phases of varieties development; the statistical variational analysis with determination of the reliability of the results; the laboratory method in which the genotyping of grape varieties was carried out by the following SSR-marker: VVS2, VVMD7, VVMD27, VVMD5, VrZAG62, VrZAG79. As a control for molecular analysis the Cabernet- Sauvignon was used in our work. The article presents the phenotypic features and DNA-passports of indigenous Daghestan varieties of Gimra and Asyl Kara and a new hybrid form obtained by hybridization of these varieties, named Fioletta, of the DSTSV&H breeding. The genetic commonality of the origin of the indigenous grape varieties studied, and the features of inheritance of phenotypic and genetic traits from parental forms are shown. It is noted in the article that the obtained DNA- passports of varieties can be effectively used to determine the clean varieties of planting material and grapes plantations of these varieties, and to determine the genetic proximity to one or another variety in the parent pair, and for the clarification of the parental forms of the sample, and also for the disputed questions of the authorship of the variety.

How to cite
Kazahmedov R., Mamedova S., Ilnitskaya E. PHENOTYPIC AND GENETIC CHARACTERISTICS OF A NEW TECHNICAL GRAPE VARIETY OF FIOLETTA OF DAGHESTAN BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2018. № 51(3). pp. 79–88. URL: http://journalkubansad.ru/pdf/18/03/08.pdf. DOI: 10.30679/2219-5335-2018-3-51-79-88 (request date: 16.12.2018).
pdf
113 Кб
5 с.
Genetic Resources, Cultivar, selection
Date posted: 15.03.2011   Informregistr code: 0421100126/0019
UDC: 634.8 : 631.52
Keywords: GRAPE, SELECTION, CLONES, PRODUCTIVITY, QUALITY, ADAPTIVE POTENTIAL

Annotation

Highly productivity protoclones of Saperavi grape are selected in the industrial plantings of grapes by method of clonal selection in the agro-climatic conditions of Taman. Protoclones characterized by high productivity of grapes, high quality vine production, have increased resistance to frost and pests

How to cite
Petrov V., Nudga T., Ilnitskaya E., Sundyreva M., Guguchkina T., Daurova E., Muzychenko A. IMPROVING OF AGROBIOLOGICAL PROPERTIES OF SAPERAVI GRAPE BY METHOD OF CLONAL SELECTION IN THE TAMAN TERRITORY [Electronic resource] // Fruit growing and viticulture of South Russia. 2011. № 8(2). pp. 12–16. URL: http://journalkubansad.ru/pdf/11/02/02.pdf. (request date: 16.12.2018).
pdf
131 Кб
7 с.
Genetic Resources, Cultivar, selection
Date posted: 15.03.2016
UDC: 575.174.015.3
Keywords: DNA-FINGERPRINTING, GRAPEVINE CULTIVARS, MICROSATELLITE PROFILE

Annotation

The aim of the present work was the DNA fingerprinting of a number of promising grapevine cultivars of NCRRIH&V and AZESV&W breeding, based on the analysis of polymorphism of microsatellite loci. The results of the study can be used for identifying of the studied cultivars and for control purebred of parent vines plantations and planting material of domestic cultivars. Domestic breeding cultivars, promising to improve the industrial assortment of grapes in the Southern Russia were studied on the molecular genetic level. The article presents the results of a DNA-fingerprinting of six domestic cultivars of grapes: Barchatny, Vladimir, Dmitry, Dostoyny, Kurchanskiy, Krasnostop AZOS. Analysis of cultivars genotypes carried out on the mixture of DNA samples of 7-10 typical plants of each cultivar. DNA profiles were obtained by microsatellite loci MD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79, these markers are recommended as the basic ones for Vitis vinifera L. genotyping. Microsatellites (SSR-simple sequence repeats) - tandem repeats of simple sequences in the DNA structure are the most often used as DNA markers for studying of the grapes genotypes. The main method used in the work - polymerase chain reaction with the separation of the reaction products using an automated genetic analyzer ABI Prism 3130. The DNA of Chardonnay and Cabernet Sauvignon were used as the control, allelic compositions of studied SSR-loci are known for these cultivars. Cultivars genotypes showed the different alleles combinations on studied loci. Thus, the minimum recommended set of microsatellite markers is sufficient to differentiate of studied genotypes. The article also provides a brief description of the studied cultivars and their main distinguishing positive agric and biological characteristics.

How to cite
Ilnitskaya E., Tokmakov S., Kaydysheva G. DNA-FINGERPRINTING OF SOME GRAPEVINE CULTIVARS OF NCRRIH&V AND AZESV&W BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2016. № 38(2). pp. 47–53. URL: http://journalkubansad.ru/pdf/16/02/04.pdf. (request date: 16.12.2018).
pdf
668 Кб
8 с.
Genetic Resources, Cultivar, selection
Date posted: 15.03.2018
UDC: 575.11: 634.8.09
DOI: 10.30679/2219-5335-2018-2-50-24-31
Keywords: GRAPEVINE, DNA-MARKERS, DOWNY MILDEW RESISTANCE, RPV10, RPV3 GENES

Annotation

Downy mildew is the one of the most prevalent diseases of the grapevine. An effective way to control the spread of disease is cultivation of resistant varieties. The European grape V. vinifera is not resistant to mildew practically. Genotypes, carrying the resistance genes, belong to the grape varieties of North America and Asia. So breeding of resistant grape varieties is based on interspecies hybridization. Modern methods of genetics and molecular biology expand the possibilities of ongoing research in this field. Today it is identified about 20 major and minor of resistance loci to P. viticola. Rpv10 and Rpv3 are major loci of resistance. Rpv10, inherited from V. amurensis was first determined F. Schwander et al. It was identified and a DNA-marker GF09-46, closely linked with this locus. Rpv3 gene was first found in the Bianca grapes a complex interspecies hybrids of grapes. It was later determined that the Rpv3 gene has seven haplotypes, and their identification is possible with microsatellite markers UDV305, UDV737. We analyzed the genotypes of selected, elite forms and new inter-species grape varieties of NCFSCHVW breeding by the PCR method using DNA markers linked to the mildew resistance Rpv10 and Rpv3 genes. The separation of the reaction products is carried out by capillary electrophoresis using an automated genetic analyzer ABI Prism 3130. The analyzes were carried out in accordance with the potential possibility of identifying the target alleles according to the pedigree of the studied samples. The Rpv3 gene is defined in six hybrid genotypes, Rpv10 gene is defined in one grape hybrid.

How to cite
Ilnitskaya E., Tokmakov S., Makarkina M. DNA MARKER-ASSISTED IDENTIFICATION OF RESISTANCE GENES TO DOWNY MILDEW IN NEW CULTIVARS AND SELECTED GRAPES FORMS BY NCFSCHVW BREEDING [Electronic resource] // Fruit growing and viticulture of South Russia. 2018. № 50(2). pp. 24–31. URL: http://journalkubansad.ru/pdf/18/02/03.pdf. DOI: 10.30679/2219-5335-2018-2-50-24-31 (request date: 16.12.2018).