Fruit growing
and viticulture of South Russia

Annotation

The use of DNA markers, along with classical identification methods based on morphological assessment, makes it possible to more accurately determine the varietal affiliation of grape plants, as well as clarify the origin of genotypes. The purpose of this work is to perform genotyping of grape varieties bred by the Dagestan Breeding Experimental Station of Viticulture and Vegetable Growing (DBESV&V) using microsatellite markers (SSR) used for varietal identification and clarify the origin of varieties using DNA analysis data. The objects of the study were six table grape varieties � Vezne, Dolchatyi, Zhemchuzhina Yuga, Zarya Derbenta, Leki and Muscat Derbenskiy. The DNA of the studied varieties was extracted by the CTAB method from herbarized plant material. Genotyping was carried out using a standard set of SSR markers: VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79 by PCR followed by analysis of the results on an ABI Prism 3130 genetic analyzer, the obtained data were processed using the GeneMapper v 4.1 program and aligned with reference variety Pinot noir. Pedigrees of varieties were analyzed based on the principle of codominant inheritance of alleles from parents to offspring. DNA profiles of parental forms (Csaba gyoengye, Muscat of Alexandria, Muscat Hamburg, Kirovabadskiy stolovyi) of the studied varieties were taken from the international database of grape varieties VIVC (Vitis International Variety Catalogue), and we also used the previously developed DNA passport of Agadai variety. As a result of the study, DNA-passports of 6 varieties of DBESV&V breeding were developed. DNA analysis confirmed origin of grape varieties Vezne, Dolchatyi, Zhemchuzhina Yuga, Zarya Derbenta, Leki and Muskat Derbentskiy from the declared parental forms.