Fruit growing
and viticulture of South Russia

Annotation

Currently, financial success in the market can be provided by varieties of fruit crops that have a complex of economically valuable and adaptively significant characteristics, at the same time, characterized by high yield capacity, proper presentation, transportability and the ability to long-term storage. The key to the cost-effective maintenance of an apple orchard is determined by rational technology, proper care of trees, and the correct selection of varieties. It is worth noting that the average yield capacity of apple fruits on the Don in the years before the reforms did not exceed 40-50 kg/ha. The low yield capacity is explained by the lack of an irrigation system in the orchards, the use seed, strong-growing rootstocks as the main, the rare standing of trees (250-300 tree/ha) in the orchard, an unsuccessful choice of varieties. The determination of the most suitable varieties for the selected climatic zone became the purpose of the research. So, in 2012, in the conditions of the Azov zone of the Rostov region, an intensive type orchard was planted on an area of 84 hectares, in which eight apple varieties of different ripening periods were placed. When laying the garden, mainly new varieties with increased resistance to diseases and pests were used. The rootstock of seedlings is weakly growing, vegetatively propagated M9 (paradizka 9, EM 9). Tree planting scheme is 3x1 m (3m2 /tree, 3333 tree/ha). In the course of our work, the fertility of these varieties, yield capacity, the influence of climate and temperature on trees, as well as resistance to diseases and pests were analyzed. Based on the obtained data, optimal varieties of various maturation periods for cultivation in the Azov zone of the Rostov region were established.

Annotation

Improving the fruit aroma is one of the promising directions of modern strawberry breeding. The strawberry fruit aroma is a complex trait, formed by a combination of a large number of volatile compounds. The present study shows the results of assessing the allelic state of the FaOMT gene, determining biosynthesis of mesifurane (volatile compound) in strawberry selected forms created in the �I.V. Michurin FSC�. The biological objects of the study were 22 strawberry selected intervarietal forms from 12 crossing combinations. The allelic state of the FaOMT gene was identified using codominant SCAR marker FaOMT-SI/NO. As a result of the studies, the marker fragment of the functional allele FaOMT+ was identified in 86.4 % of forms. A heterozygous combination of alleles of the FaOMT gene (average level of mesifurane accumulation in fruits) was detected in 9.1 % forms. The homozygous state of the functional allele was detected in 77.3% forms. The homozygous state of the non-functional allele FaOMT- (mesifurane is not produced) is characterized by three genotypes (13.6 %). The greatest value for strawberry breeding use are forms with the homozygous state of the FaOMT+ allele: 1/6-41 (Vima Zanta × Polka), 2/2-32, 2/2-41 (Faith × Tea), 3/2-62 (Vima Zanta × Privlekatelnaya), 3/4-6, 3/4-7 (Malwina × Tea), 3/9-5, 3/9-12 (Florence × Faith), 5/1-105 (Polka × Vima Zanta), 5/2-23, 5/2-26, 5/2-32 (San Andreas × Monterey), 6/3-5, 6/3-21 (Kimberly × 9/2-2), 7/2-78 (Asia × Maya), 9/2-2, 9/2-7 (Kimberly × Honeoye). These forms are characterized by the maximum level of mesifurane biosynthesis in fruits and their involvement of which in hybridization allows the transmission of the target allele to 100 % of the hybrid seedlings.

Annotation

The article provides detailed information about ampelodescriptor signs, morphological, biological and technological features of the valuable indigenous grape variety Bayanshira, originating from the ancient center of viticulture � Ganja-Dashkasan zone of Azerbaijan. The enocarpological and enochemical characteristics of bunches and berries, the population structure and the variability of this variety were studied. In order to determine the direction of use and technological suitability of the Bayanshira variety yield, an uvological assessment of bunches and berries of fresh grapes was carried out, prototypes of wines of various types were prepared and their physicochemical analysis was carried out using modern methods. As a result of assessing the intrapopulation phenotypic variability of the variety, several biotypes and clonal variations were identified, their distinctive characteristics were identified and described, and morphological features, biological and technological indicators were studied. Based on the above, a number of important indicators of the biotype of the Bayanshira variety can be noted: t he size of the bunch ranged from 17,2 × 7,7 to 26,5 × 12,6 cm, the shape of the bunches was conical, the number of berries in the bunch was generally more than 200 pieces, but only biotype 6 had an average number of berries in a bunch (150 pieces), berry size ranged from 19,4 × 19,0 to 23,4 × 22,2 mm, bush yield was 4,4-12,4 kg, yield juice from berries was 48,4-84,6 %, sugar content of berry juice was 17,4-21,4 g/100 cm3 , titratable acidity was 4,65-8.02 g/dm3 , the amount of phenolic compounds was 0,25-0,45 g/dm3 , and the amount of biologically active substances was 0,12-0,36 g/dm3 .

Annotation

The article considers the issue of scion-rootstock combinations of seedless grape varieties in Russia and the world. The rootstock influences the physiological and biochemical parameters, stability, yield capacity and quality of the yield of grafted grape varieties. It is relevant to study the experience of different countries to determine the direction of research on this topic in Russia. In the world, the main research is concentrated predominantly in the countries producing table grapes: India, China, Egypt, Chile, Brazil, Greece, there are also studies from Mexico, Chile and Australia. Depending on the agro-climatic conditions, soils and terrain, appropriate rootstocks are used. High indicators of productivity, quality and resistance to stressors can be achieved in plants of the same variety on different rootstocks, for example, the yield capacity of the Thompson Seedless variety in Egypt is higher on the 1103 Paulsen rootstock, and quality indicators are higher on the Salt Creek rootstock. In Australia, the own-root variant had a higher fruitfulness of shoots, but Thompson Seedless variety grafted on Ramsey rootstock had higher yield capacity and bunch size. Scion-rootstock combinations are studied both for proven grape varieties and for new ones. Studies of variety-rootstock combinations in Russia are limited to individual works. The main rootstock for seedless varieties is Kober 5BB. Given the high role of rootstocks in increasing the abiotic and biotic stability of plantings, increasing productivity and improving the quality of grapes, the development of research in our country in the field of studying and effective use of rootstocks is relevant.

Annotation

The paper presents the results of DNA profiling of 28 grape samples with unidentified varietal affiliation, selected from the Magarach Ampelographic Collection, and provides polymorphism assessment of their microsatellite loci. Genotyping was performed using 9 nuclear (VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79, VVS2) and 3 chloroplast (ccmp3, ccmp5, ccmp10) SSR markers. For nuclear loci, 105 alleles were identified, the average number of alleles per locus (na) was 11.7; the average effective number of alleles (ne) is 7.23; Shannon information index (I) for the studied batch of samples was 2.13. The highest allele frequency was observed in samples with VVS2133 and VVS2135 (p = 0.250), VVMD5238 (p = 0.232), VVMD7247 (p = 0.232), VVMD25239 (p = 0.250), VVMD27180 (p = 0.232), VVMD28236 (p = 0.143), VVMD32272 (p = 0.304), VrZAG62188 (p = 0.179), VrZAG79251 (p = 0.232) alleles. The average value of actual heterozygosity Heto was 0.8333, expected heterozygosity Het� was 0.8712. For chloroplast loci, 7 alleles were identified: ccmp3106, ccmp3107, ccmp5104, ccmp5105, ccmp10114, ccmp10115, ccmp10116. Also 4 chlorotypes were identified: A (1 sample), B (9), C (13), D (5). A dendrogram of genetic similarity was constructed using the UPGMA method, which showed the presence of three clusters containing, respectively, 11, 11 and 6 samples. There was a uniform distribution of chlorotypes B and D over three clusters, and chlorotype C � over two clusters. It is concluded that the results can be used to assess the genetic diversity of Magarach Ampelographic Collection.

Annotation

The results of studying the mechanical composition of bunches and berries of a young vineyard of dark-berry technical grape varieties Vecherniy, Dmitriy and Kurchanskiy are presented. The research was carried out in a standard own-root vineyard located in the first department of the Kuban educational farm of KubSAU. Data on the average mass of a bunch and berry, as well as the mass of their constituent structural elements are given: the proportion of berries and stems in the bunch weight, the proportion of skin, seeds and pulp with juice in the berry weight, the structure index of the bunch and the berry index are calculated. It was revealed that the smallest bunch weight was obtained from the Vecherniy variety, and the largest from the Kurchanskiy variety. In both years, the smallest average berry weight was observed in the Dmitriy variety, and the largest in the Vecherniy variety. The proportion of berries from the bunch weight in the studied varieties turned out to be approximately the same and amounted to 96.1�97.7 %, which was within the normal range. In both years, the structure index had the highest values in the Kurchanskiy variety, and the lowest in the Vecherniy variety. The highest values of the berry index were observed in the Dmitriy variety, which has the smallest berries, and the lowest values in the Vecherniy variety, which has the largest berries. The proportion of skin in four out of six cases was significantly higher than the standard values. The proportion of seeds from the bunch weight between varieties ranged from 5.0-9.8 %, which exceeded the norm by 1.0-3.8 %. The proportion of pulp with juice in five out of six cases was 58.2-71.7 %, which was significantly less than the standard values (75-80 %). The share of structural indicators of berries depended not only on varietal characteristics, but also on the conditions of the year.

Annotation

The article reveals the main factors and moments that characterize the develop-ment of viticulture in the Gudermessky district of the Checheno-Ingush ASSR in the 80s of the XX century. It is emphasized that this area is more suitable for viticulture than the "old" areas � Naursky, Shelkovskoy and Nadterechny. In confirmation, emphasis is placed on more favorable edaphoclimatic conditions, the possibility of introducing a non-covering and semi-covering grape culture, a higher adaptability of the population to the industry, due to the proximity to the Khasavyurtovsky District, where the population was engaged in viticulture much earlier. It was found that by the mid-1980s 8 specialized farms (wine collective farms) operated in the district, which are part of the production and collective farm association "Checheningushvino". The area of vineyards was systematically growing, the wine collective farms achieved high results in terms of gross production of grapes and grape yield capacity. A special place was given to securing vineyards for mobile groups of wine-growers (family contract) and tenants. The culture of grapes in the region occupied a prestigious place among other branches of the agro-industrial complex. It was especially noted that the processes of intensification of production were launched. High-value grape varieties with increased frost resistance were tested, allowing them to switch to a non-covering crop; the bushes were transferred to more advanced pruning, which were distinguished by a greater capacity in terms of load with shoots, etc. After the release of the Anti-Alcohol Decree and subsequent negative processes that led to the collapse of the country (and to military operations in Chechnya), the industry began to curtail. However, the experience accumulated by generations of growing grapes finds its application.

Annotation

Monitoring of the key metabolite, the amino acid proline, in the grape plant is necessary to study the physiological mechanisms underlying the adaptive reactions of grapes in a changing climate. The purpose of this work is to study the dynamics of the content of free proline in various grape varieties, to identify varieties with increased resistance to low temperatures. The objects of research are grape varieties of various genetic origin: Kristall (control), Dostoynyi, Krasnostop AZOS, Vostorg, Aligote, Zarif. The content of proline was determined by capillary electrophoresis on a Kapel 104R device according to a technique based on obtaining an electrophoregram using direct detection of the absorbing components of the sample. In January-February, the varieties Kristall, Krasnostop AZOS, Vostorg had an increased content of proline in comparison with the varieties Dostoynyi, Aligote, Zarif. In the shoots of the varieties Kristall,, Krasnostop AZOS, Vostorg, it was 7.38-8.26 �g/g fresh weight; in Dostoynyi, Aligote, Zarif � 4.12-5.16 �g/g fresh weight. In the buds of the varieties Kristall, Krasnostop AZOS, Vostorg, the content of proline was 7.92-9.21 �g/g fresh weight, in Dostoynyi, Aligote, Zarif � 4.57-5.90 �g/g fresh weight. After artificial freezing in the shoots of the varieties Kristall, Krasnostop AZOS, Vostorg, the proline content increased by 1.13-1.23 times, in other studied varieties it almost did not change. It has been shown that proline plays a significant role in the implementation of the stressprotective reactions of grapes to low temperatures. It has been established that according to the dynamics of the free proline content, the varieties Crystal, Krasnostop AZOS, Vostorg are more resistant to low temperatures in comparison with the varieties Dostoynyi, Aligote, Zarif.

Annotation

The biological activity of the soil is formed mainly due to the microbiota living in it. In this regard, when creating agricultural technologies, the properties of microorganisms are widely used to increase the biological activity of the soil and reproduce soil fertility. Harmoniously functioning soil largely determines the nutritional properties of the agricultural products produced on it, which takes the corresponding technologies from the category of regenerative to health-saving technologies. Considering that soil microorganisms play an important role in the transformation of substances in the soil, the influence of the bio-organo-mineral complex AKM on the intensity of substrate-induced soil respiration in the near-root zone of trees was evaluated to assess the degree of bioactivity. A positive effect of AKM on the growth of chlorophyll content in apple tree leaves has been established. The beginning of the increase is observed 33 days after the application of fertilizer and in subsequent periods this trend continues. The obtained results confirm the existence of a relationship between chlorophyll concentration in leaves and productivity. Based on the data of the experiment, we can conclude that the bio-organo-mineral complex AKM is a means of soil bioactivation. This is expressed in an increase in soil respiration during the growing season by 8.8-45.5 %. The magnesium contained in the complex provides an increase in suction power due to an increase in the concentration of chlorophyll (4.8-36.5 %) and the intensity of photosynthesis, which ultimately leads to an increase in the availability of mineral nutrients associated with fruit trees. As a result of the action of A�M, fruit yield capacity increases by 14.3 %, and their quality also increases. The importance of using magnesium fertilizers, in particular AKM, is associated not only with the ability to increase crop yields, but also with the ability to produce physiologically more valuable products in terms of nutrient content, which can be used in health conservation programs for the prevention, including endemic diseases of the population.

Annotation

Modern agriculture is impossible without the use of plant protection products against pests and diseases, which, with their free development, significantly reduce the productivity of plantings and the marketability of the harvested crop. Research conducted in this direction abroad is aimed at using predictive mathematical models that link environmental factors that are significant for the pest and its development. At the same time, the system of intellectualization of such a forecast is increasingly being used with the use of artificial intelligence methods that independently form models based on constantly accumulating information databases. The aim of the study was to evaluate the effectiveness of an automated program for forecasting the development of the oriental fruit moth, developed on the basis of the temperature factor of the environment for the subsequent selection of an optimal adaptive plant protection system for perennial crops. To compare the results of the automated calculation of the development of the eastern fruit moth with objective data in the field, we carried out observations of the calendar dates of the butterfly flights and overcoming the economic threshold of harmfulness (ETH) on pheromone traps placed in peach plantations, on which chemical treatments with insecticides were not carried out in the period from 2019 to 2022. In the course of the research, a useful computer program was developed to determine the calendar dates of the development of the oriental fruit moth based on air temperature data using flexible logical algorithms. It is established that the variability of the terms of individual development of the pest provides an increase in the phase periods with each successive generation. The use of a computer program will allow us to develop a system for protecting trees from pests, as well as to adjust the timing of measures depending on objectively changing indicators of the conditions of the year.

Annotation

The paper presents the results of studies of trophic relationships of Lobesia botrana Den. et Schiff. (Lepidoptera, Torticidae) in the �parasitoid - insect-host� system in ampelocenoses. For the first time for the Anapa-Taman viticulture zone of the Krasnodar region, the species Bassus tumidulus (Nees, 1812) (Microdus tumidulus (Nees von Esenbeck, 1812), Therophilus tumidulus (Nees von Esenbeck, 1812) was established as a mass natural enemy of the European grape moth in the conditions of industrial plantings of grapes. During the observation period (2021-2023), this type of parasitoid was the most common in terms of the number of infected specimens of L. botrana, its frequency of occurrence exceeded that of other parasitoids recorded in these studies by 2.7 times. There is a lack of knowledge of the parasitoid fauna in the consortia formed around the European grape moth in ampelocenoses. Knowledge of trophic relationships of the main economically significant pest of the vineyards of the Anapa-Taman zone of the Krasnodar region as a mechanism for regulating its abundance can help in the development of effective biologized protection measures in the fight against it. According to the results of extirpation, the seasonal dynamics of the flight of the adult parasitoid was revealed and compared with the life cycle of the harmful lepidopteran host. A complete adaptation of Bassus tumidulus to the target phases of the development of the European grape moth has been established. The conclusion is made about the specialization of the polyphage to this pest in the region. The characteristic features of Bassus tumidulus population dynamics were revealed, namely, an increase in the density of the hymenoptera population in ampelocenosis by the end of the growing season. The data of the biology of Bassus tumidulus, given in the literature, have been confirmed.

Annotation

One of the most important tasks in the production of high-quality red wines is the preservation of intense color, which exposed to atmospheric oxygen, can change and acquire garnet or brown shades. The intensity of color, its shades and stability are determined by the presence of phenolic compounds in wine, among which polymeric forms of polyphenols and anthocyanins have a rich color. In addition, the phenolic substances of red wines are represented by monomeric forms, catechins and procyanidins, etc. The impact of oxygen at various stages of the technological process of making wine can have both positive and negative effects on its chemical composition and organoleptic characteristics. The purpose of this research was to study the effect of oxygen introduced at various technological stages of the preparation of wine materials. As a result of studies of the indicators of the mass concentration of oxygen and phenolic substances that cause wine oxidation, as well as the organoleptic indicators of dry red wines (production samples) made from the Cabernet Sauvignon grape variety in various geographical zones of southern Russia, significant differences have been revealed due to agro-climatic conditions of growing grapes in a particular area, as well as the grape processing technology used at enterprises. Under the conditions of the laboratory and production unit �Micro wine-making�, the possibility of using the microoxidation method both at the stage of must and pulp fermentation, and after the fermentation process (stabilization and clarification) for the purpose of deliberate oxidation of polyphenols, was shown. Based on the conducted studies, the optimal dosage of exogenous oxygen, which has a positive effect on the organoleptic characteristics of wine materials, is a concentration of 4-6 mg/dm3 both during microoxidation at the stage of must and pulp fermentation, and at the end of the fermentation of wine.

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The paper discusses methods for identifying wines based on regional characteristics, which consists of establishing a connection between the component composition of wines and soils corresponding to the area where grapes grow. It has been shown that markers of regional affiliation can be components whose content in wines practically does not change during the production process. The results of a study of the mineral composition of wines produced by the winemaking enterprise OOO �Imenie Sikory� are presented. Using atomic absorption spectroscopy using the Kvant Z spectrometer (NPF OOO �KORTEK�, Russia), 12 metal elements (Co, Mn, Rb, Cr, Cs, Al, Ba, Zn, Sr, Li, Na, Mg) were determined in the course of studies on atomic emission and absorption spectra. A study was also conducted of the mineral composition of soil samples from vineyards where grapes used for wine production grew. Using the methods of correlation and cluster analysis, as well as using the �random forest� algorithm (for wine samples, the initial data were the content of elements and their pairwise ratios), it was revealed that the mineral composition can serve as a marker of the regional affiliation of wine products, since the studied wine samples are within each variety have a stable mineral composition, regardless of the year of harvest (correlations between the year and the content of elements are insignificant), determined by the characteristics of the site where the grapes grow. Quantitative determination of the components of the mineral complex in combination with mathematical processing of the results allows us to establish the origin of the wines with a reasonable degree of probability. The mineral composition of the studied wines is unique and is determined by the soil characteristics of the vineyard areas of the �Semigorye� geographical object.

Annotation

Currently, the production of high-quality sparkling wines is one of the main tasks in wine-making. The indicator of foamy and sparkling properties of finished sparkling wines directly depends on the ability of the base wine to foam. This indicator is influenced by various factors: the grape variety, the composition of the wine material, the agrotechnological and technological methods used. The main purpose of the study was to study the influence of varietal characteristics of grapes on the foaming ability of wine materials produced in the Krasnodar region in the 2022 season. All the original samples were processed and fermented under identical conditions. The study of the component composition of the samples was carried out according to standard methods using modern equipment. To determine the ability of foam formation by the initial samples, a "Foaming analyzer" was used, developed by Mishin M.V. on the basis of the Department of Technology of Wine-making and Fermentation named after Professor A.A. Merzhanian, which allows an objective assessment of the quality of foam. The foaming ability of three introduced grape varieties was analyzed: Pinot Blanc, Riesling Rhenish and Chardonnay. The result of the study was the establishment of a connection between the foaming ability of wine materials and the grape variety. In the course of the study, it was found that all the samples under study, both in terms of chemical composition and organoleptic evaluation of the aroma, were suitable for creating sparkling wine. However, according to the totality of the composition (esters, higher alcohols and amino acids), wine materials from the Riesling Rhenish variety had the greatest potential for the formation of foaming capacity for the 2022 season in the Temryuk district of the Krasnodar region.

Annotation

An important condition for the development of the food industry is the use of digital technologies in calculations to reduce the time and cost of production. The relevance of the topic is due to the importance of studying the prerequisites for ensuring the digital development of food industry enterprises for the food security of the state. An analysis of advanced foreign experience in the digital development of the food industry was carried out. The directions of digital development of the food industry based on the use of robotization technologies for sorting raw materials, digital twins, computer vision, artificial intelligence, analysis of a large volume of data, visual systems for machine assessment of the quality of finished products and others are noted. The introduction and development of automated programs in technological processes and modeling of recipe compositions has become very relevant in recent years. This is due to the complexity of the calculation due to the large number of main and auxiliary raw materials used, which increases the volume of calculations and production costs. To solve such problems, it is necessary to use modern computer technologies and digitalize processes. The analysis of literature data has shown that the modeling of food products using digital technologies is carried out in the chosen direction: vitamin and mineral composition and energy value. Particular attention is paid to the design of food products and the creation of optimal formulations from plant raw materials that meet the specified requirements of target groups of consumers. The most popular software products for the processing industry in Russia are of a specialized nature, which allow automating and combining the development of technological and production documentation at each stage of the process into a single system for managing the quality and safety of food products from the input quality of raw materials to the sale of finished products. The use of digital technologies in the calculation of food formulations will allow solving the problems of multi-corrective optimization. It is concluded that it is necessary to form a digital format for the development of the food industry in ensuring the quality and safety of food products.

Annotation

In order to expand the range of flour confectionery products for a healthy diet, the possibilities of using cheap and affordable plant raw materials rich in nutrients have been studied. The main raw material is flour from light-grain rye of the Pamyati Bambysheva variety of the FASC of the South-East breeding. As a substitute for white sugar, powder from sugar beet root crops grown in the Balakovo district of the Saratov region was used. The evaluation of organoleptic indicators of the quality of products was determined according to GOST 14621-78, and a tasting assessment of the quality of finished products was carried out, quality indicators were evaluated on a 9-point scale. Physico-chemical parameters were determined according to generally accepted methods: the mass fraction of sugar � according to GOST 5903-89, the mass fraction of fat � according to GOST 31902-2012, ash content � according to GOST 5901-2014, humidity of products � according to GOST 5900-2014. The foaming ability, density and stability of the whipped mass were determined in the work. As a result of research, it can be concluded that when wheat flour is replaced with flour from light-grain rye of the Pamyati Bambysheva variety, a pleasant smell and taste are noted in the finished biscuit semi-finished product, according to physico-chemical indicators, the mass fraction of sugar and fat decreases, which gives the products dietary properties. The nutritional and energy value of the biscuit semi-finished product changes in a positive direction, as the carbohydrate content decreases, and the content of polyunsaturated omega-3 and omega-6 fatty acids, vitamins, macro- and microelements, many of which are antioxidants, increases markedly. Therefore, the results of these studies make it possible to position the finished product as a product with functional and dietary properties.