Thematic electronic scientific online journal NCFSCHVW

Fruit growing
and viticulture of South Russia



Kiseleva Galina


Candidate of Biological Sciences, associated professor

Articles in journal: (total 32)

pdf
980 Кб
14 с.
Physiology and biochemistry of plants
Date posted: 27.03.2023
UDC: 581:576.5:634.224
DOI: 10.30679/2219-5335-2023-2-80-156-169
Keywords: GRAPES, ELECTROPORETIC SEPARATION OF PEROXIDASES, STABILITY, HEAT, DROUGH

Annotation

The search for reliable methods for assessing the resistance of grape varieties to heat and drought is relevant in connection with the increasing climate instability in the Anapo-Taman viticulture zone. The purpose of the work is to conduct electrophoretic separation of peroxidases in polyacrylamide gel in the leaves of various grape varieties, to identify varieties with increased resistance to heat and drought. The objects of research are grape varieties of various ecological and geographical origin: Kristall (control) Euro-Amur-American origin; Dostoynyi, Krasnostop AZOS Euro-American origin; Vostorg Amur-American origin; Zarif Eastern origin; Aligote Western European origin. It was established that the qualitative and quantitative composition of peroxidase isoforms changed during the summer and had a varietal feature. In June, 4-6 isoforms were isolated for each particular variety, in August 2-4 isoforms. The Kristall variety stood out for its especially high resistance to heat and drought during the summer, both in the field and under the influence of artificial high-temperature and water stresses. This was achieved due to the multiple isoform composition of peroxidases and the constancy of their composition in response to stress. Varieties Dostoynyi, Vostorg, are highlighted as highly resistant to heat throughout the summer. The heat resistance of varieties Krasnostop, Aligote, Zarif changed during the summer period. In relation to drought, the varieties Kristall, Dostoynyi, Vostorg showed high resistance in early and mid-summer. According to the appearance and disappearance of peroxidase isoforms, the drought resistance of varieties Dostoynyi, Vostorg, Krasnostop AZOS changed during the summer. Aligote and Zarif varieties during the summer period stood out as not drought-resistant. The obtained data can be used as diagnostic criteria for assessing the degree of heat and drought resistance of grape varieties in different months of the summer period in the conditions of the Anapo-Taman zone of the Krasnodar region for breeding purposes.

How to cite
Kiseleva G., Ilina I., Sokolova V., Zaporogets N., Luzkii E., Vyalkov V. THE USE OF PEROXIDASE ISOFORMS TO IDENTIFY GRAPE VARIETIES RESISTANT TO HEAT AND DROUGHT [Electronic resource] // Fruit growing and viticulture of South Russia. 2023. № 80(2). pp. 156–169. URL: http://journalkubansad.ru/pdf/23/02/11.pdf. DOI: 10.30679/2219-5335-2023-2-80-156-169 (request date: 05.05.2024).
pdf
1034 Кб
28 с.
Date posted: 28.03.2024
UDC: 634.8: 551.58
DOI: 10.30679/2219-5335-2024-2-86-16-43
Keywords: GRAPES, METHODS, MANAGEMENT, SUSTAINABILITY, PRODUCTIVITY, QUALITY, FORECASTING

Annotation

The article presents the results of agrobiological and physiological-biochemical studies to ensure the stability of grape plantations in unstable weather conditions and anthropogenic intensification of production. Studies in the agroecological conditions of the Krasnodar region on the southern chernozems revealed the most productive norm of loading of grape bushes with shoots for the technical varieties Kurchanskiy, Dmitriy, Vladimir, Granatovyi, Antaris and Alkor, on leached chernozems for the table seedless variety Centennial Seedless. According to the results of electrophoretic separation of peroxidases in polyacrylamide gel and an increased content of ascorbic acid, increased frost resistance was established in the varieties Kristall, Vostorg, Krasnostop AZOS. The Vostorg and Zarif varieties proved to be more adaptive to the stresses of the summer period in terms of leaf hydration, bound water content, the ratio of chlorophylls and carotenoids, the amount of proline and water-soluble sugars, membrane permeability. The role of physiologically active substances in increasing the adaptive potential of grape plants has been established. The treatment of Merlot vines with methyl jasmonate and epibrassinolide during deep dormancy period, as well as proline, salicylic acid and epibrassinolide during induced dormancy period had a positive effect on the condition of the vine: there was a decrease in electrolyte yield. Sustainable grape cultivation is achieved with the systemic use of the Bioconcentrate-Z effluent by the non-root method. Against the background of nonroot treatments of Merlot and Cabernet Sauvignon varieties, a higher level of plant water supply, an increase in potassium content, green pigments, organic acids and grape yields were established. Physiologically active compounds and the preparation Turinbash have a positive effect in intensive own-root plantations of native and promising introduced grape varieties against the background of phylloxera infection in the southern part of Dagestan. The created yield forecasting method based on the NDVI of the grape plant and the actual data of multispectral images of the phenophases of flowering and growth allows to increase the accuracy of the calculated yield capacity to the actual one with a deviation from 0 to 0.8 kg per bush, the accuracy of the forecast increases by 2 times by the end of the growth phase and by the beginning of the maturation phase.

How to cite
Petrov V., Aleynikova G., Seghet O., Marmorshtein A., Russo D., Sundyreva M., Kiseleva G., Kazahmedov R., Orlov V. METHODOLOGY OF MANAGEMENT OF AGROBIOLOGICAL, ADAPTIVE AND PRODUCTIVE STABILITY OF GRAPE PLANTATIONS IN UNSTABLE WEATHER CONDITIONS AND TECHNOGENIC INTENSIFICATION OF PRODUCTION [Electronic resource] // Fruit growing and viticulture of South Russia. 2024. № 86(2). pp. 16–43. URL: http://journalkubansad.ru/pdf/24/02/02.pdf. DOI: 10.30679/2219-5335-2024-2-86-16-43 (request date: 05.05.2024).