Fruit growing
and viticulture of South Russia

Annotation

The sterilization of explants is the most important step for the successful plants cultivation in vitro culture. The disinfectant have to neutralize the pathogenic microflora and do not damage of the plant tissues. This article present the assessment of the effectiveness of using the chlorine-containing tablets of the OKA-TAB trademark as an sterilizing substance for the rehabilitation of explants. One tablet of disinfectant contains 1.41-1.87g of active chlorine. For the illustrative treatment 0,5 % solution was used. The exposure time was 5 minutes. The presence of contamination, damage to the plant tissues (necrosis of objects) and the output of viable explants were taken into account. Apexes of SK 7, SK 2, SK 3, M 9, MM-106 apple rootstocks were introduced in vitro culture. Initiation was carried out during the active growth of shoots of apple rootstocks (May-June). Apexes were planted on a nutrient medium culture supplemented with the salts according to the prescription of Murashige and Skoog media (1962): ascorbic acid � 1 mg /l, vitamins of B1, B6 and PP � 0.5 mg /l, mesoinositol � 100 mg /l, sucrose � 30 g and agar-agar � 0,8 %, BAP � 0,4 mg /l. The plants were cultivated with the photoperiod of 16 hours, with air temperature of + 24 � 2 �C and illumination of 2-3 thousand lux. According to the study, it was found out that 0,5 % solution of chlorine-containing tablets at an exposure of 5 minutes had a higher efficiency. The yield of explants was 75-98 % depending on the genotype. Therefore, OKA-TAB tablets could be used for surface treatment of apple rootstock explants during the active period of shoot growth, as an alternative remedy to sublimate (0,1 %).

Annotation

The results of evaluation of short stratification periods with different durations influence the obtaining healthy microplants of sweet cherry hybrids from isolated unripe embryos in vitro culture are presented. Three sweet cherry varieties of super early, early and meddle term of ripening are studied. The sterilization scheme for immature sweet cherry embryos using a disinfectant in the form of tablets containing the sodium salt of dichloroisocyanuric acid was tested. The method showed the high efficiency, which was manifested in a low percentage of infected tubes. Three seeding were made at various times: after harvesting immediately, after 1 week, and after 3 weeks of stratification. The degree of plants development introduced in vitro culture was defined after 2, 3, and 5 weeks. Assessment of plants with root and leaves was carried out on a 5-point scale. Based on the development degree of plants introduced in vitro culture, a graph was created showing that the highest percentage of normally developing embryos of the Krasnodarskaya Rannya sweet cherry was observed in the group stratified for three weeks, than that stratified one week. In the group of the Krasa Kubani specimens, an opposite dependence is observed. The rate and degree of development of the Yaroslavna embryos without stratification are in the middle range between other studied samples with long and short stratification. Based on the data obtained, it can be concluded that the varietal characteristics of samples prevail over the duration of stratification in order to increase in the yield of normally developed microplants obtained in vitro embryo culture.

Annotation

Control of varietal purity of planting material is a significant factor affecting the productivity of horticultural farms. SCoT markers (Start Codon Targeted) can be attributed to modern methods for monitoring the varietal purity of seedlings of fruit crops, which have the prospect of being introduced into wide practice. In this connection, based on the prospects of using SCoT markers in the identification of plant material, work was carried out to select effective combinations of SCoT markers. An approach based on the creation of markers from two SCoT primers was used to increase the number of possible marking options. The aim of this work was to select combinations of SCoT mark-ers that are promising for apple DNA fingerprinting, which in the future will allow the best combinations to be used in the identification of planting material. The paper presents the results of the evaluation of combinations of SCoT-markers to identify representatives of the genus Malus. The 18 SCoT markers were grouped into 68 combinations based on the preliminary assessment. The selection of effective combinations of markers was carried out according to a number of necessary criteria, such as the high quality of DNA finger-prints and a significant number of polymor-phic amplified DNA fragments. These characteristics are especially significant in the genetic assessment of closely related genotypes. The requirements for the quality of DNA fingerprints increase significantly, due to the large number of identical DNA fragments. About 45 % of the combinations were successful and were selected for fur-ther work as promising. The approbation carried out in the work made it possible to identify promising markers among 68 used ones. Selected DNA markers can be useful in a variety of areas, including the genetic identification of accessions, the analysis of the genetic homogeneity of plants obtained through micropropagation, as well as for performing research on the study of the genetic relationships of accessions.

Annotation

The production of Russian wine is based on the use of active dry yeast imported into the Russian Federation from European countries � France, Germany, Italy. Meanwhile, it is known that the high-quality wines of a geographical name must be produced using the local yeast races. The search for such races (strains) is an urgent task. Analysis of the genetic diversity of wild yeast populations and the creation of their collections is a necessary initial step in creating the new promising strains for industrial winemaking. Such studies are ongoing in Europe to create terroarspecific yeast strains. Grapes were sampled to isolate and study the new local strains of wine yeast in the vineyards of Temryuk and Anapa districts of the Krasnodar Territory. DNA isolation, conditions for PCR and fragment analysis were carried out according to current methods. To analyze the polymorphism length of amplified fragments during SSR genotyping, a fragment analysis was used on an ABIprism 3130 automated genetic analyzer. The data obtained were visualized in Gene Mapper v 4.1. A variety of species and genera of yeast on the surface of grape berries has been established. Moreover, the �yield� of saccharomycetes amounted to about 30 % of the total number of monospore cultures obtained for each of the selection points. The results of the analysis of genetic Relationships and a number of technological characteristics of strains of Saccharomyces sp. Isolated from natural population in ampelocenoses are presented. It was revealed that the geographical factor influenced the occurrence of genetic isolation. The genetically removed strains display the significant differences in a number of physiological and biochemical characteristics.

Annotation

Recently, the task of updating the walnut assortment with new adaptive and high-quality varieties of domestic breeding has been solved, since foreign varieties cannot fully realize their biological potential in the conditions of the North Caucasus. In order to solve the issue of import substitution, it is necessary to create competitive, highly productive varieties with lateral fruiting, adaptive to unfavorable factors. At the same time, an extremely important characteristic of the variety is the quality of the fruit. The article presents the results of research on the search for promising forms of walnut. During the route trips, a preliminary assessment of the studied samples was carried out for the presence of lateral fruiting and fruit load, as a result of which 11 promising forms were identified for assessing the morphometric characteristics and quality of the fruits. All 11 selected forms of walnut are valuable for breeding and can serve as sources of economically useful traits (lateral fruiting, large fruit, high percentage of kernel yield, light, well-filled kernel, thin shell). Forms � 1, � 3, � 4, � 5, � 6, � 7, �. 8, � 10 can be considered the most promising in terms of the complex of phenotypic characteristics of fruits. These samples have an average (� 1, � 5, � 7, � 8) and high (� 3, � 4, � 6, � 11) percentage of kernel yield, good kernel extractability, kernel completion and can be used in breeding work, such as sources of economically valuable traits.