Fruit growing
and viticulture of South Russia

Annotation

Downy mildew is one of the most common and harmful diseases of the grapevine, caused by the biotrophic oomycete Plasmopara viticola. The disease causes significant damage to the grape harvest and gets worse its quality. One of the most effective methods to control the disease is the cultivation of resistant grape varieties. The grape varieties Vitis vinifera (the basis of the modern high-quality assortment) practically have not the genetic resistance to this disease. Downy mildew resistant genotypes belong to the North American and Asian grape varieties, as well as Muscadinia rotundifolia. The search for resistance donors and their inclusion in the process of creating the new highly resistant genotypes is an important task of modern breeding and genetics. Today, more than 20 loci of resistance to downy mildew have been identified in the genome of grapes. Rpv12 gene was identified by S. Venuti et al. using QTL analysis. A set of DNA markers associated with the Rpv12 gene was also detected and recommended for DNA-marker breeding. The donor of this resistance gene is wild Vitis amurensis. We conducted a PCR study of the grape genotypes � potential gene carriers, according to their pedigree, using DNA markers (UDV343, UDV360) linked to the downy mildew resistance gene Rpv12. The separation of the reaction products was made by capillary electrophoresis using an automatic ABI Prism 3130 genetic analyzer. For DNA-markers testing, DNA of the Kunleany and Zarya Severa varieties was included in the work. According to the literature, the Rpv12 gene is presented in these varieties. Also, the varieties that do not carry the resistance gene (Cabernet Sauvignon and Chardonnay) were included in the work as negative controls. Targeted PCR products are identified in the Kunleany and Zarya Severa genotypes, that corresponds to the literature data, as well as in the genotypes Stepnyak and Vostorg.

Annotation

Powdery mildew of grapes or oidium is one of the most common and economically significant diseases of grapes. The causative agent of the disease is biotrophic ascomycete Erysiphe necator. Powdery mildew is a global disease that reduces the yield and quality of grapes, while causing significant damage to viticulture around the world. Most cultivated grape varieties are susceptible to this pathogen, therefore, pesticide treatment is used to reduce the incidence of disease. Resistance to powdery mildew is mainly found in the genotypes of North American and Asian grape varieties. The search for donors of resistance to powdery mildew and the subsequent creation of new resistant and high-quality grape varieties is one of the main tasks of grape breeding. Cultivation of resistant varieties will reduce the use of pesticides, which in the future will make it possible to switch to more environmentally safe industrial viticulture. At present, a number of loci of resistance to powdery mildew (more than 10) have been identified, and DNA markers have been constructed that are suitable for identifying the allelic status of these genes. Among the identified loci of resistance to powdery mildew, Ren3 and Ren9 genes have a large contribution. The study involved elite technical forms of grapes of the NCFSCHVW breeding, the genotypes of which could potentially contain loci of resistance to powdery mildew (Tana 19, Tana 72, Tana 73, Tana 74, Tana 82, Tana 92). To identify the genes that cause genetic resistance to powdery mildew, specific codominant SSR markers GF15-42, ScORGF15-02 were used to identify the Ren3 gene, and SSR marker CenGen6 to identify the Ren9 gene. As a positive control, we used the DNA of the cultivar Seyve Villard 12-375, which has resistance alleles. The work was carried out by the PCR method, with the separation of reaction products using the method of capillary electrophoresis on an automatic genetic analyzer Nanofor 05. As a result of DNA marker analysis, it was determined that the genotype Tana 92 contain the Ren9 gene. The Ren3 and Ren9 genes has been identified in grapevine Tana 73 and Tana 74.

Annotation

Molecular markers make it possible to identify varieties, study their origin, and identify synonyms, homonyms, and impurities in the collections. DNA passports of grape varieties present the genotype profiles for a set of microsatellite (SSR) loci. SSR markers VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79 are the minimum basic set in the work on DNA-passportization of grape varieties. Using this indicated set of SSR markers, we genotyped the varieties of Barkhatny, Dostoyny, Krasnostop AZOS, Krasnostop Anapskiy and Phillokseroustoychivy Dzhemete grapes from the Anapa Zonal Station of Viticulture and Winemaking and also of the Muscat Hamburg variety included in the study as one of the parental forms of Barkhatny and Dostoyny varieties. The main method, which was used in the work, is polymerase chain reaction (PCR) with the separation of reaction products on an ABI Prism 3130 automated genetic analyzer. DNA of the Chardonnay and Cabernet Sauvignon reference varieties was used as a control to refine the sizes of identified alleles. DNA was isolated by the CTAB method from the apical leaves of young shoots. Genotype analysis was carried out on DNA from a mixture of plant material of 3-5 typical plants for each variety. The genotypes of the studied varieties showed the different combinations of alleles on the studied microsatellite loci. As a result of DNA profiles analysis, we revealed that the Dostoyny grape variety has a different origin, namely the Muscat Hamburg is not its parent form, at the same time it was confirmed that the other parent form is Phillokseroustoychivy Dzhemete. The obtained DNA profiles of varieties can be used to identify them, verify the variety pure of uterine plantation and planting material, and protect the author's rights.

Annotation

When analyzing the state of the viticulture industry in Russia, there is a lack of seedless grape varieties in the assortment that have valuable biological and economic characteristics and a short growing season, resistance to frost and phylloxera. Due to the advantage of seedless grapes both for fresh consumption and for the production of dried products, processing them into jams and other products, the demand for kishmish varieties is growing and seedless varieties are regularly selected. The degree of various varieties seedlessness can be different: from the almost full absence of rudiments in the berry to a sufficiently noted size of rudiments. At the same time, the degree of development of seed rudiments in the same variety may vary in dependence on the conditions of the growth place and the year conditions. We analyzed the harvest of 33 seedless grape varieties in the Ampelographic collection (Anapa) according to the evaluation of their seedless under the agro-ecological conditions of the Black Sea area of viticulture under the climatic conditions, 2019. The lack of recoverable rudiments of seeds was identified in the berries of cultivars the Kishmish Bely Ovalny, Detskiy, Kishmish Tarakli, Kishmish Rosovy, Kishmish Sogdiana, Kishmish Krugly, Remali Seedless. The maximum berry`s weight among this group was found in the variety of Kishmish Sogdiana. The largest rudiments were found in the berries of the Rusbal variety. Among all analyzed grape varieties, the rudiment of the berry`s mass of which has been measured, it is possible to select the Kishmish Luchisty: this variety has the highest average weight of berries (5.05 g) and the rudiments share of the berry`s mass is 1 %.

Annotation

Analysis of existing grapes assortment in the Krasnodar region and its structure and tendencies of change is presented. The problem of improvement of grapes assortment in unstable environment conditions is presented. Varieties of local breeding for assortment improvement are offered