Fruit growing
and viticulture of South Russia
Phytosanitary condition of plants
The grapevine (Vitis vinifera L.) is one of the most important fruit crops in the world. Cultivated grape varieties are considerably affected by a large number of pathogenic microorganisms that cause diseases during the growing season impacting the quantity and quality of the crop its processing and export. Potential threats include bacteria, fungi, oomycetes or viruses with different life cycles, infection mechanisms and survival strategies. In recent years there has been an increase in grape fusarium and a change in the complex of its pathogens in various countries. Fusarium of grapes being a relatively new and dangerous disease negatively affects grape must. A complex of fungi of the genus Fusarium is involved in the defeat of grapes the main feature of which is the ability to produce mycotoxins. The species composition and the proportion of each species in the complex of detected fungi can vary with changes in the conditions of cultivation or storage of grapes, which is accompanied by changes in the spectrum of mycotoxins. Therefore, effective Fusarium control methods need to be introduced in the field before harvest especially using alternative methods to pesticides such as biocontrol. One of the most promising but at the same time poorly developed is the use of antagonists of mycotoxin producers. The use of yeast fungi as a biologized technology of protection against Fusarium in viticulture is relevant. Frequently reported yeast antagonists include strains belonging to the genus Pichia, Meyerozyma, Rhodotorula, Metschnikowia Saccharomyces, Candida, Hanseniaspora, and the yeast-like fungus Aureobasidium pullulans. The review is devoted to the characterization of Fusarium fungi pathogenic for grapes and perspectives of application of biological means of their control with the help of yeast fungi.
In the protection of fruit crops from a complex of pathogens, one of the current tasks is to prevent the development of root rot, among the causes of which are the activity of soil micromycetes Phytophthora sp. Selective media play a key role both in identification and subsequent storage in the laboratory, and in the study of morphological and cultural traits, sporulation ability, as well as to obtain a certain amount of inoculum (for example, for in vivo studies) and mycelium (for DNA isolation). Obtaining a pure culture of pathogenic micromycete with certain morphological and cultural characteristics, which may be important for diagnosis by both traditional and modern methods, determines the relevance of studying the influence of various media on these parameters of the pathogenic fungus Phytophthora sp. The studies were carried out by methods of laboratory and comparative analyses. The growth of the pathogen strain of apple root rot Phytophthora sp. on nine nutrient media was studied. The colony growth rate and morphological and cultural characteristics were evaluated. As a result of the research, the strain has been found to vary not only the growth rate of the colony, but also the cultural characteristics depending on the nutrient medium. Of the nine nutrient media, three universal ones were selected that are most suitable for cultivation and identification, namely, tomato, potato-glucose and must agars. They meet the following criteria: ensuring the maximum degree of sporulation, rapid growth and development of the mycelium of the fungus, ease of preparation. It was also noted that Leroux medium can be used to obtain a large amount of inoculum, since it will produce more spores when planted in a lawn than on other media.
Modern agriculture is impossible without the use of plant protection products against pests and diseases, which, with their free development, significantly reduce the productivity of plantings and the marketability of the harvested crop. Research conducted in this direction abroad is aimed at using predictive mathematical models that link environmental factors that are significant for the pest and its development. At the same time, the system of intellectualization of such a forecast is increasingly being used with the use of artificial intelligence methods that independently form models based on constantly accumulating information databases. The aim of the study was to evaluate the effectiveness of an automated program for forecasting the development of the oriental fruit moth, developed on the basis of the temperature factor of the environment for the subsequent selection of an optimal adaptive plant protection system for perennial crops. To compare the results of the automated calculation of the development of the eastern fruit moth with objective data in the field, we carried out observations of the calendar dates of the butterfly flights and overcoming the economic threshold of harmfulness (ETH) on pheromone traps placed in peach plantations, on which chemical treatments with insecticides were not carried out in the period from 2019 to 2022. In the course of the research, a useful computer program was developed to determine the calendar dates of the development of the oriental fruit moth based on air temperature data using flexible logical algorithms. It is established that the variability of the terms of individual development of the pest provides an increase in the phase periods with each successive generation. The use of a computer program will allow us to develop a system for protecting trees from pests, as well as to adjust the timing of measures depending on objectively changing indicators of the conditions of the year.
The paper presents the results of studies of trophic relationships of Lobesia botrana Den. et Schiff. (Lepidoptera, Torticidae) in the parasitoid - insect-host system in ampelocenoses. For the first time for the Anapa-Taman viticulture zone of the Krasnodar region, the species Bassus tumidulus (Nees, 1812) (Microdus tumidulus (Nees von Esenbeck, 1812), Therophilus tumidulus (Nees von Esenbeck, 1812) was established as a mass natural enemy of the European grape moth in the conditions of industrial plantings of grapes. During the observation period (2021-2023), this type of parasitoid was the most common in terms of the number of infected specimens of L. botrana, its frequency of occurrence exceeded that of other parasitoids recorded in these studies by 2.7 times. There is a lack of knowledge of the parasitoid fauna in the consortia formed around the European grape moth in ampelocenoses. Knowledge of trophic relationships of the main economically significant pest of the vineyards of the Anapa-Taman zone of the Krasnodar region as a mechanism for regulating its abundance can help in the development of effective biologized protection measures in the fight against it. According to the results of extirpation, the seasonal dynamics of the flight of the adult parasitoid was revealed and compared with the life cycle of the harmful lepidopteran host. A complete adaptation of Bassus tumidulus to the target phases of the development of the European grape moth has been established. The conclusion is made about the specialization of the polyphage to this pest in the region. The characteristic features of Bassus tumidulus population dynamics were revealed, namely, an increase in the density of the hymenoptera population in ampelocenosis by the end of the growing season. The data of the biology of Bassus tumidulus, given in the literature, have been confirmed.
The article presents the actual results of observations on the prevalence of sugescent pests in the Rostov region, the dynamics of abundance, population, and the peculiarities of the phenological development of pests on technical varieties, depending on meteorological conditions. It is noted that an air temperature of +14 ... +30 ºC and a relative humidity of 52 % are necessary to maintain viability and fertility. The eggs of the buffalo ceresa leafhopper overwinter in the branches and trunks of trees of woody species, often in young annual shoots. The female ovipositor cuts the bark and lays eggs in two rows of 5-11 eggs. Damage by the buffalo ceresa leafhopper causes a violation of the flow of nutrients to the tops of shoots, weakening and a sharp decrease in their growth. The rose leafhopper gives up to 4 generations during the summer. Damaged leaves turn yellow, photosynthesis processes are disrupted in them, plant nutrition deteriorates, which affects the reduction of economically valuable indicators. The presence of sucking pests increases the risk of spreading viral diseases. Insecticides and phytosanitary monitoring are most effective in fruit-bearing vineyards only in combination with high agricultural techniques of cultivation. In this connection, there is a decrease in yield losses and environmental pollution with chemicals. Therefore, it is necessary to deal with weeds in a timely manner, to carry out maintenance work to ensure good ventilation of grape bushes, to regularly carry out phytosanitary monitoring of the population with sugescent pests and diseases, to carry out row-to-row tillage. Chemical treatments are necessary only if the threshold number of pests is exceeded. The article provides suggestions on how to contain the number of pests at a low level to obtain an environmentally safe grape yield.