Fruit growing
and viticulture of South Russia

Annotation

Improving the fruit aroma is one of the promising directions of modern strawberry breeding. The strawberry fruit aroma is a complex trait, formed by a combination of a large number of volatile compounds. The present study shows the results of assessing the allelic state of the FaOMT gene, determining biosynthesis of mesifurane (volatile compound) in strawberry selected forms created in the �I.V. Michurin FSC�. The biological objects of the study were 22 strawberry selected intervarietal forms from 12 crossing combinations. The allelic state of the FaOMT gene was identified using codominant SCAR marker FaOMT-SI/NO. As a result of the studies, the marker fragment of the functional allele FaOMT+ was identified in 86.4 % of forms. A heterozygous combination of alleles of the FaOMT gene (average level of mesifurane accumulation in fruits) was detected in 9.1 % forms. The homozygous state of the functional allele was detected in 77.3% forms. The homozygous state of the non-functional allele FaOMT- (mesifurane is not produced) is characterized by three genotypes (13.6 %). The greatest value for strawberry breeding use are forms with the homozygous state of the FaOMT+ allele: 1/6-41 (Vima Zanta × Polka), 2/2-32, 2/2-41 (Faith × Tea), 3/2-62 (Vima Zanta × Privlekatelnaya), 3/4-6, 3/4-7 (Malwina × Tea), 3/9-5, 3/9-12 (Florence × Faith), 5/1-105 (Polka × Vima Zanta), 5/2-23, 5/2-26, 5/2-32 (San Andreas × Monterey), 6/3-5, 6/3-21 (Kimberly × 9/2-2), 7/2-78 (Asia × Maya), 9/2-2, 9/2-7 (Kimberly × Honeoye). These forms are characterized by the maximum level of mesifurane biosynthesis in fruits and their involvement of which in hybridization allows the transmission of the target allele to 100 % of the hybrid seedlings.

Annotation

The article provides detailed information about ampelodescriptor signs, morphological, biological and technological features of the valuable indigenous grape variety Bayanshira, originating from the ancient center of viticulture � Ganja-Dashkasan zone of Azerbaijan. The enocarpological and enochemical characteristics of bunches and berries, the population structure and the variability of this variety were studied. In order to determine the direction of use and technological suitability of the Bayanshira variety yield, an uvological assessment of bunches and berries of fresh grapes was carried out, prototypes of wines of various types were prepared and their physicochemical analysis was carried out using modern methods. As a result of assessing the intrapopulation phenotypic variability of the variety, several biotypes and clonal variations were identified, their distinctive characteristics were identified and described, and morphological features, biological and technological indicators were studied. Based on the above, a number of important indicators of the biotype of the Bayanshira variety can be noted: t he size of the bunch ranged from 17,2 × 7,7 to 26,5 × 12,6 cm, the shape of the bunches was conical, the number of berries in the bunch was generally more than 200 pieces, but only biotype 6 had an average number of berries in a bunch (150 pieces), berry size ranged from 19,4 × 19,0 to 23,4 × 22,2 mm, bush yield was 4,4-12,4 kg, yield juice from berries was 48,4-84,6 %, sugar content of berry juice was 17,4-21,4 g/100 cm3 , titratable acidity was 4,65-8.02 g/dm3 , the amount of phenolic compounds was 0,25-0,45 g/dm3 , and the amount of biologically active substances was 0,12-0,36 g/dm3 .

Annotation

The article considers the issue of scion-rootstock combinations of seedless grape varieties in Russia and the world. The rootstock influences the physiological and biochemical parameters, stability, yield capacity and quality of the yield of grafted grape varieties. It is relevant to study the experience of different countries to determine the direction of research on this topic in Russia. In the world, the main research is concentrated predominantly in the countries producing table grapes: India, China, Egypt, Chile, Brazil, Greece, there are also studies from Mexico, Chile and Australia. Depending on the agro-climatic conditions, soils and terrain, appropriate rootstocks are used. High indicators of productivity, quality and resistance to stressors can be achieved in plants of the same variety on different rootstocks, for example, the yield capacity of the Thompson Seedless variety in Egypt is higher on the 1103 Paulsen rootstock, and quality indicators are higher on the Salt Creek rootstock. In Australia, the own-root variant had a higher fruitfulness of shoots, but Thompson Seedless variety grafted on Ramsey rootstock had higher yield capacity and bunch size. Scion-rootstock combinations are studied both for proven grape varieties and for new ones. Studies of variety-rootstock combinations in Russia are limited to individual works. The main rootstock for seedless varieties is Kober 5BB. Given the high role of rootstocks in increasing the abiotic and biotic stability of plantings, increasing productivity and improving the quality of grapes, the development of research in our country in the field of studying and effective use of rootstocks is relevant.

Annotation

The paper presents the results of DNA profiling of 28 grape samples with unidentified varietal affiliation, selected from the Magarach Ampelographic Collection, and provides polymorphism assessment of their microsatellite loci. Genotyping was performed using 9 nuclear (VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79, VVS2) and 3 chloroplast (ccmp3, ccmp5, ccmp10) SSR markers. For nuclear loci, 105 alleles were identified, the average number of alleles per locus (na) was 11.7; the average effective number of alleles (ne) is 7.23; Shannon information index (I) for the studied batch of samples was 2.13. The highest allele frequency was observed in samples with VVS2133 and VVS2135 (p = 0.250), VVMD5238 (p = 0.232), VVMD7247 (p = 0.232), VVMD25239 (p = 0.250), VVMD27180 (p = 0.232), VVMD28236 (p = 0.143), VVMD32272 (p = 0.304), VrZAG62188 (p = 0.179), VrZAG79251 (p = 0.232) alleles. The average value of actual heterozygosity Heto was 0.8333, expected heterozygosity Het� was 0.8712. For chloroplast loci, 7 alleles were identified: ccmp3106, ccmp3107, ccmp5104, ccmp5105, ccmp10114, ccmp10115, ccmp10116. Also 4 chlorotypes were identified: A (1 sample), B (9), C (13), D (5). A dendrogram of genetic similarity was constructed using the UPGMA method, which showed the presence of three clusters containing, respectively, 11, 11 and 6 samples. There was a uniform distribution of chlorotypes B and D over three clusters, and chlorotype C � over two clusters. It is concluded that the results can be used to assess the genetic diversity of Magarach Ampelographic Collection.

Annotation

The results of studying the mechanical composition of bunches and berries of a young vineyard of dark-berry technical grape varieties Vecherniy, Dmitriy and Kurchanskiy are presented. The research was carried out in a standard own-root vineyard located in the first department of the Kuban educational farm of KubSAU. Data on the average mass of a bunch and berry, as well as the mass of their constituent structural elements are given: the proportion of berries and stems in the bunch weight, the proportion of skin, seeds and pulp with juice in the berry weight, the structure index of the bunch and the berry index are calculated. It was revealed that the smallest bunch weight was obtained from the Vecherniy variety, and the largest from the Kurchanskiy variety. In both years, the smallest average berry weight was observed in the Dmitriy variety, and the largest in the Vecherniy variety. The proportion of berries from the bunch weight in the studied varieties turned out to be approximately the same and amounted to 96.1�97.7 %, which was within the normal range. In both years, the structure index had the highest values in the Kurchanskiy variety, and the lowest in the Vecherniy variety. The highest values of the berry index were observed in the Dmitriy variety, which has the smallest berries, and the lowest values in the Vecherniy variety, which has the largest berries. The proportion of skin in four out of six cases was significantly higher than the standard values. The proportion of seeds from the bunch weight between varieties ranged from 5.0-9.8 %, which exceeded the norm by 1.0-3.8 %. The proportion of pulp with juice in five out of six cases was 58.2-71.7 %, which was significantly less than the standard values (75-80 %). The share of structural indicators of berries depended not only on varietal characteristics, but also on the conditions of the year.