Fruit growing
and viticulture of South Russia
The relevance of the work lies in the necessity of replacing animal proteins (gelatin and its analogs, fish glue) with vegetable proteins in the processing of wine materials in order to clarify and stabilize them. The influence of potato protein on clarification and physical and chemical parameters of white, rose and red table and liqueur wine materials was investigated. The optimal technological dosages of potato protein were established using trial fining in comparison with the generally accepted gelatin treatment. It was shown that the optimal technological dosages of potato protein, which provided the desired transparency of wine materials, were 2-4 times higher in comparison with gelatin. Dosages of bentonite for complex technological processing were almost identical for gelatin and potato protein. The dynamics of clarification of wine materials was different: when gelatin was added, opalescence was formed in the processed wine materials, followed by aggregation and sedimentation of the sludge. The rapid formation of large aggregates and their settling were noted in case of application of potato protein in wine materials. The volume and weight of sludges in case of using potato protein were 10-15% more in comparison with gelatin. However, the sludges formed during the processing of wine materials with potato protein were denser and less mobile. With an increase in the dosage of potato protein, the turbidity and color intensity of wine materials decline, the mass concentration of phenolic compounds, the sum of colloids and the reduced extract decrease. The volume fraction of ethyl alcohol, mass concentrations of titratable and volatile acids, organoleptic indicators do not undergo significant changes. Based on the studies carried out, the application of potato protein for the processing of wines is recommended.
The possibility and feasibility of creating functional food products is considered. It has been shown that grapes can and should be used as an antioxidant with dietary and therapeutic properties. The modern ideas about the formation of food rations, which imply the need to take into account the individual characteristics of the physiology of people for whom the product will be developed, are considered. Abnormalities of the human body, such as oxidative stress and metabolic syndrome, are among the main objects of control. In developing a scheme for analyzing functional ingredients of grapes, the main regulatory provisions of international standards, guidelines of the Codex Alimentarius Commission, Russian standards, reference data on the chemical composition of Russian food products were taken into account. The analysis of the available information base, the use of which makes it possible to establish the functionality of a food product and/or an ingredient for a functional purpose, has been carried out. A table of contained in grape nutrients that allowed to select the desired composition of grapes has been developed. The work was carried out on the example of the available and obtained properties of resveratrol and flavonoids, sufficiently mastered and recommended compounds of the vine with the studied chemical composition and structure. For the development and systematic use of food products with proven properties, following concepts are disclosed: Functional ingredients and functional products. Three samples of semi-finished products were obtained from the Rylines pink seedlings grape variety: juice, must, juice with the addition of mint. The samples were tested for the content of polyphenols and ascorbic acid. According to the test results, it was determined that juices have the best biochemical parameters for functional purposes. Using the obtained results, on the basis of proven quality control techniques, the feasibility of developing a new methodology for creating demanded and controlled indicators of a recommended for development functional food product was determined.
The grape-growing industry is a priority in the agro-industrial complex of Daghestan. At present, when special attention is paid to creating conditions for the production of "protected geographical indication" wines, it is necessary to expand the research of grape raw materials used for their production. In this regard, in the Republic of Daghestan the study of nutritional and technological properties of new autochthonous grape varieties, along with introduced ones is actual, to recommend the production of wines from them that can increase the prestige of local wine products. It is determined that the qualitative composition and quantitative content of pools of aroma-producing substances in red and rose wine materials made from grapes of technical local varieties Gimra Novaya and Fioletta, as well as the Pervenets Magaracha (harvest 2019), grown in the conditions of the Derbent district of the Republic of Daghestan on the plantation of the Dagestan Selecting Testing Station of Viticulture and Horticulture branch of the Federal State Budget Scientific Institution North Caucasian Federal Scientific Center of Horticulture, Viticulture, Winemaking. Aroma-producing substances were determined on a gas chromatograph Crystal 2000M (RF). A significant amount of carbonyl compounds (325.48 mg/dm3 ) and esters (252.94 mg/dm3 ) was found in the rose wine material produced from the Pervenets Magaracha and Fioletta varieties. The highest total number of higher alcohols (1871.37 mg/dm3 ) and aromatic components (2588.68 mg/dm3 ) was found in the wine material from the red variety Gimra Novaya. A high concentration of 2,3 butylene glycol and a higher alcohol, isoamyl alcohol, was noted. The total mass concentration of volatile acids in the studied experimental samples of wine materials was 439.56-781.28 mg/dm3 . It is determined that the natural conditions of the southern zone of Daghestan are favourable for the cultivation of grapes of autochthonous varieties Gimra Novaya, Fioletta and introduced variety Pervenets Magaracha. Using them as raw materials for obtaining high-quality wine materials will allow you to form in their bouquet the tones of smoky prunes, hints of the taste of pomegranate and blackthorn fruits.
The mass concentration of biogenic amines is an important indicator of the quality and safety of food products, including alcohol products. Biogenic amines are formed as a result of the decarboxylation of free amino acids by enzyme systems of microorganisms. It means that their formation has a biotechnological nature and is directly related to the vital activity of microorganisms. Contact of wine materials with microorganisms yeast and malolactic bacteria, can lead to an increase in the concentration of amino acids and provoke the formation of biogenic amines. In this regard, the purpose of this research was to study changes in the concentrations of biogenic amines and amino acids in the process of alcohol and malolactic fermentation. Conducted studies have shown that the total concentration of biogenic amines in the grape must of both white and red grape varieties was minimal. Biogenic amines methylamine and putrescine were not detected. It was found that as a result of alcohol and malolactic fermentation, the concentration of biogenic amines increased and the corresponding amino acids decreased. At the same time, the total concentration of all the studied amino acids in wine materials after alcohol fermentation increased, and after malolactic slightly decreased, because part of the yeast cells was autolyzed during secondary fermentation of the wine materials. At the same time, mass exchange processes between the cell and the medium were activated, leading to enrichment of the wine material with amino acids. Thus, favorable conditions were created for the activation of decarboxylases, as a result of which there was an increase in the concentration of biogenic amines. The obtained data allow to suggest that in order to reduce the level of biogenic amines in the finished product, special attention must be paid to the selection of a race of yeast and malolactic bacteria that are unable to produce biogenic amines or introduce them in minimal amounts.
This article proposes a technology for the production of grape spirits from a sweet grape pomace, which consists in fermentation of the grape pomace using a pure growth of yeast, adding yeasty grounds and water, a piquette separation, its distillation with the selection of the head and tail fractions and the secondary distillation to obtain a 94-95 % grape rectified spirit by volume. The distillation of the resulting raw materials was performed on the installation of a LUMMARK consisting of a distiller and a distillation column. The prepared piquettes were subjected to primary distillation using the DV-3 distillator, distillates (raw spirit) were distilled on the RUM-3 distillation column with electrical heating. Primary distillation mode: the volume of the piquette taken to distillation is 50 l, the heating mode before the distillation starts at the power of the electric heater 3kW (55-65 min). The rectifying distillation mode: the volume of the raw alcohol is 40 l, the heating mode before the selection of the head fraction is started with the power of the electric heater 3kW (40-55 min). In the preparation of raw materials to primary distillation, it was established that the optimal duration of the fermentation of raw materials for the production of piquettes in order to reduce the content of volatile acids, methanol and other secondary fermentation products that have a negative effect on finished products should not exceed 4 days. It has been established that to obtain high-quality raw spirit, it is necessary to carry out the selection of head fractions in an amount of at least 3 % of the volume for all raw materials, for distillate from grape must of technical varieties of grapes at least 1.5 % of the volume. It is shown that the organoleptic and physicochemical indicators of rectified grape spirits, produced by the proposed technology, corresponded to the standards established in the standard regardless of the type of raw materials (wine grape, table grape).