Fruit growing
and viticulture of South Russia
The paper presents the results of a study of the biofunctional state of agrogenically altered soils of two phytocenoses: naturally-like (cypress plantations) and agrocenosis (peach orchard). The relevance of the systemic biomonitoring of soils for various agricultural use is made. The main goal of the work was determined to assess the state of brown forest soils of various phytocenoses according to a set of indicators in order to understand their informative value and the relationship between them. The experimental plots were located on brown forest soils in the humid subtropical climate of the Black Sea coast. The number of representatives of three morphological groups of saprotrophic microbocenosis was analyzed along the horizons of the soil profile. The soil for research was selected in the winter, to study the biological activity of soils, the spring period was chosen. The analysis of the number of microorganisms and indicators of the ecophysiological state was carried out in relation to the agrochemical parameters of soils and weather conditions. The physiological group of saprotrophic bacteria predominated in the structure of the microbial community, the number of actinomycetes and micromycetes was lower. The arable layer under the peach orchard was characterized by lower values of the number of micromycetes in comparison with the conventional background (cypress plantations). At the same time, the indicators reflecting the potential functional activity of microbial cenosis were higher in the soils of the peach agrocenosis in comparison with cypress plantations, which demonstrated the balance of the composition of the functionally active populations of this microbial community. In general, the studied complex of indicators of the biofunctional state of soils demonstrates their information content and interrelation, which allows us to consider them as indicators of the ecological state of soils for agricultural use.
The influence of systemic application of top-dressing for Chardonnay and Merlot grapes with water solutions of special organomineral microfertilizers the biological productivity of plants, the chemical composition of berries and the quality indicators of wine materials is studied. In 2016-2017, special organomineral composition containing amino acids, ascophyllum nodosum algae extract and the trace elements (B, Cu, Fe, Zn, Mn, Mo, Co) were used in the Black Sea zone of the Krasnodar Territory. Significant influence of agricultural ways the number of fruit-bearing shoots, inflorescences and growth activity of grapes was revealed. In comparison with the control variant (without preparations), the number of fruit-bearing shoots per bush increased by 7.4-19.2 % and inflorescences by 3.7-12.5 %. In the summer period, against the background of the negative effect of abiotic factors, sufficient hydration of cells in grape plants with preparation`s treatments was achieved by increasing in the connected form of water. The photosynthetic activity of the leaves was maintained due to the formation of a more powerful palisade mesophyll. Fertilizing with organomineral microfertilizers helped to strengthen the reproductive function of plants (an increase in yield by 19-30 %, depending on the variety), and had a positive effect on the commercial quality of grapes. In the juice of grapes a sugar content is increased in comparison with the control variant by 17 % for Chardonnay and 14 % for the Merlot, and as a result, the volume fraction of ethyl alcohol in wine was significantly higher compared to control samples (1.3% vol.). Top dressing with organomineral microfertilizers contributed to a decrease in the mass concentration of titrated acids in the juice of grape berries by 13 %. A higher organoleptic value of wine materials from grapes grown on the background of organomineral top dressing was given. The samples had more complete varietal nuances and long good taste.
One of the limiting factors of consistently high yields with good quality are various diseases of the crop. For example, diseases caused by phytoplasmas can have a serious negative impact the quantity of the crop and its quality, which can further affect the quality of wine products and profits. Phytoplasmas are one of the most dangerous phytopathogens. On grapes, they are represented by two species Candidatus Phytoplasma vitis Flavescence dorée (causes Golden yellowing of grapes) and Candidatus Phytoplasma solani Bois noir. Many plant diseases that are thought to be caused by phytoplasmas were described before molecular genetic studies identified the various groups of phytoplasmas that cause these diseases. It is now possible to assess the relationship between the classification of phytoplasmas and specific plant diseases. Golden yellowing of leaves is a quarantine disease for the Russian Federation and the European Union and it causes a great damage to vineyards, so quick and accurate identification of this disease is very important. The main method for identifying phytoplasmas is real-time PCR (RT-PCR) with specific primer systems. The aim of our study was to compare the methods for extracting DNA from plant tissue affected by phytoplasma for further real-time PCR. This study showed that the samples isolated using the commercial kits "AgroDiagnostics" and "CytoSorb" show the similar results, while the sample isolated by the laboratory method based on the use of CTAB buffer showed higher and earlier peaks on the graph, which is important for detecting a small amount of pathogen in the test material and proves the greater effectiveness of this method of isolation.
Downy mildew is a vine disease caused by the obligate heterothallic biotrophic endoparasite Plasmopara viticola. The area of distribution of this pathogen are vineyards all over the world, but the greatest losses are in the viticulture zones with a temperate continental and subtropical climate. In Krasnodar Territory, epiphytotic development of mildew occurs 6-7 times in 10 years. The first molecular genetic studies of the pathogen began at the end of the 20th century. The purpose of this work is to evaluate, based on DNA marker analysis, the polymorphism of the P. viticola population in two generations of the pathogen on grape plants growing in an isolated geographic point. The material for the study was the affected grape leaves taken from the vegetation plot of the FSBSI NCFSCHVW, from plants without chemical treatment. The material was taken in two time intervals the end of July (the first generation of the pathogen 4 samples) and the middle of August (the second generation 6 samples). To study the diversity, highly polymorphic SSR-markers GOB and PV144, were used. P. viticola DNA was isolated directly from infected leaves by the CTAB method. A total of 10 DNA samples of the pathogen were analyzed. The study was carried out by the classical method of polymerase chain reaction. The size of the target fragments of the PV144 and GOB loci was estimated using an ABI Prism 3130 automatic genetic analyzer by fragment analysis. The data obtained were analyzed using Gene Mapper 4.1 software. The highest degree of polymorphism was found for the GOB marker 7 types of alleles and to a lesser extent PV144 4 types of alleles. It was found that the samples of the pathogen population collected during the first generation have significantly higher genetic polymorphism, in contrast to the samples collected during the second generation. Research in this direction continues.
The production of competitive geographical denomination wines, that can solve import substitution issues and improve the quality of wines, should become one of the promising direction for the development of Daghestan wine-making. In this regard, there is a need to produce the high-quality wines from grape varieties with high biological value The production of wines raising the prestige of the Republic, fixed under the term wines of protected geographical names, must also be supported, as well as the use of local grape varieties as well as raw materials for their production, along with introduced ones. It is made a comparative study of experimental samples of wine materials, obtained from grapes of local technical the Gimra Novaya, Fioletta varieties, and introduced variety of Pervenets Magaracha, grown in the Southern plain zone of Daghestan. Physical and chemical parameters were determined by standard methods used in anoxemia. The study of phenolic substances and vitamins was carried out using capillary electrophoresis on the "Kapel 104T" device. It was found that the wine material from the Gimra Novaya grape variety significantly exceeds other experimental samples in the amount of phenolic substanced (3361 mg/dm3 ), content of ascorbic (100.13 mg/dm3 ), orotic (27.45 mg/dm3 ), caffeic (29.90 mg/dm3 ), gallic acids (38.80 mg/dm3 ) and the total amount of biologically active substances (226.89 mg/dm3 ). The results of research carried out have revealed that the local grape varieties of Gimra Novaya and Fioletta have some advantages in terms of the content of biologically active substances, compared to the introduced variety of Pervenets Magaracha and can be used for the preparation of high-quality extraordinary red and pink blended wines.