Fruit growing
and viticulture of South Russia
The article considers the issue of the impact of the load of table grape bushes with shoots and bunches on plant productivity. The place of research is the fourth subzone of the Central Agroecological Zone of viticulture of the Krasnodar region, characterized by a moderate continental climate and lowhumus, leached powerful chernozems as soils. As an object of research after exploratory studies in 2021, among different table grape varieties (Gurman Kraynova, Dubovskiy rozovyi, Kishmish Dubovskiy and Centennial Seedless), the Centennial Seedless variety was selected as promising for studying the load of bushes with shoots and bunches according to the parameters of the proportion of fruiting shoots (93 %), the bunch weight (0.73 kg) and the productivity of the shoot (0.73 kg per shoot). According to the indicator complex in the first year of research after the experimental field trial, the following variants of bush loading with shoots and bunches were distinguished: the control first variant (35 shoots and 27 bunches per bush after breaking-off shoots) by the proportion of dead buds (16.7 %) and fruiting shoots (72.5 %), the number of inflorescences (30 pcs.), the fruitfulness coefficient k1 (0.75), yield capacity (26.7 t/ha) and shoot productivity (0.59 kg per shoot); the fourth variant (23 shoots and 21 bunches) according to the fruitfulness coefficient k1 (0.81) and the productivity of the shoot (0.7 kg per shoot), the fifth variant (23 shoots and 15 bunches) according to the fruitfulness coefficient k2 (1.45), the bunch weight (0.91 kg), the loss from the bush (0.33 kg) and the productivity of the shoot (0.56 kg per shoot), the seventh variant (17 shoots and 15 bunches) according to the proportion of dead buds (16 %), fruitfulness coefficients k1 (0.81) and k2 (1.55); the ninth variant (13 shoots and 8 bunches) according to the proportion of fruiting shoots (61.5 %), fruitfulness coefficient k1 (0.77) and shoot productivity (0.55 kg per shoot).
The article presents the results of studies of the degree of favorability of agroecological conditions of the South-coast zone of Crimea for growing grapes. The long-term data on weather stations of the Crimean peninsula are analyzed. The following climatic indices describing the conditions of vegetation and ripening of grapes are calculated: the sum of temperatures above 20 ºC, the Winkler and Huglin indices, the ratio of the sum of temperatures above 20 ºC and 10 ºC, the average air temperature during the growing season, the Selyaninov hydrothermal coefficient, the amount of precipitation per year and during the growing season. Agroecological factors limiting the possibility and efficiency of grape cultivation are also analyzed: the sum of active temperatures above 10 ºC, the average of the absolute minima of air temperature. With the help of geoinformation modeling, a digital complex map of the spatial distribution of index data on the analyzed territory was constructed. The distribution of territories in the Southern coast of Crimea that are not subject to the planting of vineyards is analyzed: with unfavorable soil conditions, with a height of more than 600 m above sea level, with a slope of more than 20 degrees, as well as lands of forest and nature reserves. As a result of a comprehensive analysis of agroecological conditions in the territory of the Southern coast of Crimea, 15 ampeloecotopes were identified, including: on the territory of the city district of Alushta 7, the city district of Sudak 11, the city district of Feodosia 8, the city district of Yalta 5. As a result of comparing the agroecological conditions of the selected ampeloecotopes with the requirements of grape varieties for growing conditions, taking into account the dependence of the quality indicators of viticultural and wine-making products on agroecological factors, recommendations on agroecological optimization of varietal composition and terroir specialization of the viticultural and wine industry in the Southern coast of Crimea were developed.
Creation and cultivation of economically feasible and environmentally safe orchard, especially important in a market economy. An important task for expanding the area of cultivation of subtropical fruit crops, in particular, oriental persimmon, is to increase the frost resistance of varieties and obtain planting material with a well-developed root system. In the fruits of subtropical crops (persimmon, fig, feijoa, black olive) with valuable medicinal and dietary properties, the sugar content (mainly fructose) is several times higher than that of conventional fruit crops (apple, pear, plum). Oriental persimmon fruits contain 0.025-0.0415 mg % of vitamin P, 0.15-11.8 mg % of carotenoid, l100-530 mg% of leucoanthocyanin, 45.6 mg% of vitamin . Three types of persimmons have become widespread in Dagestan, - date plum persimmon with a well-developed fibrous root system, common persimmon with high frost resistance and oriental persimmon with high commodity fruit qualities. The idea to combine all three types of persimmon in one plant using vegetative hybridization was implemented in work: date plum persimmon (rootstock), oriental persimmon (scion) and common persimmon (intercalary insert). The resulting three-component planting material meets high requirements and can be used in regions with relatively cold climates. Practically, when growing such persimmon plants in areas with a cold climate, there is no need for transportation valuable fruits from the southern regions and, accordingly, the price will decrease and the quality indicators of this product will remain. Breeding studies are planned in the future with the participation of these oriental persimmon plants with intercalary insert of common persimmon.
The development of horticulture requires the maximum realization of the biological potential of productivity by plants. The cultivation of perennial crops on high-carbonate soils in the south of Russia and, in particular, the Crimea, limits the increase in productivity by the weak availability of macro- and microelements contained in the soil. Domestic horticultural science has moved in the direction of the selection of carbonate-resistant rootstocks and the application of plant topdressing. Abroad, on similar soils, at the same time, the possibility of reducing carbonate content by using sulfur-containing ameliorants is being studied. This direction has been little studied in our country and requires detailed consideration in model or production conditions. The field trial of studying the effect of chemical sulfur-containing ameliorant in preparing the soil for laying the orchard was carried out in the period from 2014 to 2020. At the same time, not only granulated sulfur was used as options, but also as an event generally accepted in the region the introduction of large doses of phosphogypsum. Also, as a measure for a faster transition of sulfur to the active state, one of the options was with sowing mustard in the rows of the orchard for subsequent grinding and embedding in the soil. Due to the introduction of granular sulfur for plantage treatment, a decrease in soil carbonate was achieved. At the same time, the application of phosphogypsum had no effect on the reaction of the soil solution. The use of phosphogypsum as a chemical ameliorant positively affected the productivity of perennial plantings by 35%, however, it is possible to achieve an increase in yield capacity through the application of sulfur followed by sowing mustard in row spacing (the increase in the first yield compared to the control is 105 %).
Plum pox virus in stone fruit crop plantings is one of the factors increasing the cost of cultivation of these crops. Infestation of stone fruit crops can reach 41-50 % in the Russian Federation regions of the middle zone, which is a serious threat to the production process. Infection with this virus often causes drop of leaves, ovaries and fruits in susceptible varieties. Methods to combat this virus are expensive and difficult to implement in the technological process. The most effective approach is plantation monitoring and elimination of infected plants. Since PPV contains RNA in its structure, the assay must obtain a quality preparation of total RNA. To obtain information on the quality of the isolated RNA, internal positive controls of RT-PCR are often used. In this work, the method for identification of the plum pox virus using a duplex with an internal amplification control was perfected. The ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene was used as a control because of its stable expression and high mRNA copy number. The material for this study was leaves of Kabardinskaya ranniaya plum (Prunus domestica L.), collected in early and late May in Krasnodar (Krasnodar region). The analysis was performed using molecular biological methods of reverse transcription and polymerase chain reaction. Detection of amplification products was performed in 2% agarose gel. Plum pox virus was diagnosed in all examined samples of symptomatic material. Multiplex PCR with the primer pairs under study required the use of gradient annealing of the primers with a temperature change of 3.18 C/sec. When using a cDNA matrix less than 50 ng/l, a decrease in PCR quality is possible. A method for the diagnosis of plum pox virus using an internal amplification control was perfected and modified.